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Product Info Summary
| SKU: | M07078 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, IP, IF, IHC, WB |
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Product info
Product Name
Anti-Keratin KRT6C Monoclonal Antibody
SKU/Catalog Number
M07078
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Keratin KRT6C Monoclonal Antibody (Catalog # M07078). Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Keratin KRT6C Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07078)
Host
Mouse
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Monoclonal
Clone Number
Clone: C11
Isotype
IgG1
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with purified human cytoskeletal preparations from A431 cells.
Reactive Species
M07078 is reactive to KRT6C in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
60.0 kDa
Background of KRT6C
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. There are two types of keratins (cytoskeletal and microfibrillar) and are clustered in a region of chromosome. Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (i.e. skin) and non-keratinizing cells (i.e. mesothelial). Anti-Keratin Antibody is useful for researchers interested in cytoskeletal signaling and developmental biology research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07078 is guaranteed for ELISA, IP, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:20,000
IHC: 1:50 - 1:200
IF Microscopy: 1:50 - 1:200
IP: 1:100
WB: 1:50 - 1:200
Anti-Keratin Antibody has been tested in ELISA, immunohistochemistry, immunofluorescence, immunoblotting and immunoprecipitation. For a positive control use skin, colon carcinoma and squamous granulocyte carcinoma cells.
Validation Images & Assay Conditions
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Immunohistochemistry of Mouse anti-Keratin antibody. Tissue: human prostate. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-Keratin antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Staining: Keratin as precipitated red signal with hematoxylin purple nuclear counterstain.
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Immunofluorescence Microscopy of Boster Immunochemical's Anti-Keratin antibody was used with Boster's DyLight™ 488 goat anti-mouse [shown in green] to detect Keratin by Immunofluorescence. In the same experiment, Boster's polyclonal Anti-HDAC-1 antibody was used with Atto425 Anti-Rabbit IgG [shown in red] to detect HDAC-1. Data was collected on a STED-CW TCS-SP5 Confocal system (Leica Microsystems) equipped with a DFC 350FX camera allowing sequential acquisition in wide-field, confocal and STED CW imaging modes and provided courtesy of: Myriam Gastard, PhD, personal communication, Leica Microsystems, Inc. USA
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Western blot using Boster Immunochemical's Mouse Anti-Keratin antibody. This antibody recognizes a single 56kDa band corresponding to human keratin as confirmed by the position of molecular weight markers (not shown). Approximately 100ng of keratin from human epidermis was applied under reducing conditions to a pre-cast 4-20% iGel from Gradipore Inc. A 1:400 dilution of Mab anti-Keratin was used for 2h followed by detection using a 1:5,000 dilution of IRDye™800 conjugated Goat-a-Mouse IgG [H&L] and visualization using the Odyssey® Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.
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Immunofluorescence using Boster Immunochemical's Mouse Anti-Keratin antibody. Confocal slices of HeLa cells are between 0.5 and 0.6 µm where the image is taken near the bottom of the cell. Use FITC conjugated Goat-a-Mouse IgG [H&L] at 1:2,000 dilution for detection.
Specific Publications For Anti-Keratin KRT6C Monoclonal Antibody (M07078)
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Customer Q&As
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7 Customer Q&As for Anti-Keratin KRT6C Monoclonal Antibody
Question
Would anti-Keratin Monoclonal antibody M07078 work on dog IHC with ectocervix?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
Our lab technicians have not validated anti-Keratin Monoclonal antibody M07078 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Keratin Monoclonal antibody M07078 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog ectocervix in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-22
Question
I see that the anti-Keratin Monoclonal antibody M07078 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-07-04
Question
Would anti-Keratin Monoclonal antibody M07078 work for IP with ectocervix?
L. Moore
Verified customer
Asked: 2017-03-29
Answer
According to the expression profile of ectocervix, KRT6C is highly expressed in ectocervix. So, it is likely that anti-Keratin Monoclonal antibody M07078 will work for IP with ectocervix.
Boster Scientific Support
Answered: 2017-03-29
Question
We are currently using anti-Keratin Monoclonal antibody M07078 for human tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
V. Mangal
Verified customer
Asked: 2017-02-21
Answer
The anti-Keratin Monoclonal antibody (M07078) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-02-21
Question
See below the WB image, lot number and protocol we used for ectocervix using anti-Keratin Monoclonal antibody M07078. Please let me know if you require anything else.
W. Edwards
Verified customer
Asked: 2016-07-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-07-08
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ectocervix using anti-Keratin Monoclonal antibody M07078. Let me know if you need anything else.
M. Baker
Verified customer
Asked: 2015-05-18
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-05-18
Question
I would like to test anti-Keratin Monoclonal antibody M07078 on human ectocervix for research purposes, then I may be interested in using anti-Keratin Monoclonal antibody M07078 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Dhar
Verified customer
Asked: 2013-02-01
Answer
The products we sell, including anti-Keratin Monoclonal antibody M07078, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-02-01
