This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
MKI67 antibody (M00254-11) performs well in IF, IHC, ICC.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
Filter:
IF analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (M00254-11).
Ki67/MKI67 was detected in a paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated at 1:50 rabbit anti-Ki67/MKI67 Antibody (M00254-11) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Ki67/MKI67 was detected in a paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated at 1:50 rabbit anti-Ki67/MKI67 Antibody (M00254-11) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Bundle Save
Anti-Ki67/MKI67 Rabbit Monoclonal Antibody
MKI67 antibody
| Host | Application |
|---|---|
| Rabbit | IF, IHC, ICC |
Tested Applications
MKI67 antibody was positively detected by IHC in:
human colon cancer tissue, mouse spleen tissue, rat spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
MKI67 antibody was positively detected by IF in:
human intestine cancer tissue
MKI67 antibody was positively detected by ICC/IF in:
A549 cell
Recommend Dilution
Application
Dilution
Species
IHC 1:50-1:200
IF 1:50-1:200
ICC/IF 1:50-1:200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
IHC
IF
Human
Tested
Tested
Mouse
Tested
Recommend
Rat
Tested
Recommend
Product Information
Product Name
Anti-Ki67/MKI67 Rabbit Monoclonal Antibody
View all MKI67 Antibodies
View all MKI67 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Isotype
Rabbit IgG
Immunogen
Recombinant protein with human Ki67 (Position: K2860-I3256).
Calculated Molecular Weight
358.7 kDa
Cite This Product
Anti-Ki67/MKI67 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00254-11)
Protocols
Immunohistochemistry (IHC) protocol for MKI67 antibody
Immunofluorescence (IF) protocol for MKI67 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for MKI67 antibody
Publications
Application:
Species:
Association between type 2 diabetes mellitus and colorectal adenoma: A retrospective study with insights into Wnt/β-Catenin/TCF7L2 pathway activation
Published: 2026-02-27
Journal: World Journal of Gastrointestinal Surgery
Application: IHC
Impact Factor: 1.70
Species: Human
PMID: 41809341
Effect of circadian rhythm disruption on benign prostatic hyperplasia in rats
Published: 2025-10-15
Journal: PeerJ
Application: IHC
Impact Factor: 2.40
Species: Rat
PMID: 41112764
Review
Have you used Anti-Ki67/MKI67 Rabbit Monoclonal Antibody?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-Ki67/MKI67 Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00254-11
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026