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Product Info Summary
| SKU: | A10858-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-KIR5.1 KCNJ16 Antibody
SKU/Catalog Number
A10858-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-KIR5.1 KCNJ16 Antibody catalog # A10858-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KIR5.1 KCNJ16 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10858-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from mouse Kir5.1. AA range:369-418
Reactive Species
A10858-1 is reactive to KCNJ16 in Human, Mouse, Rat
Calculated molecular weight
47.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10858-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of HeLa cells using KIR5.1 Polyclonal antibody.
Specific Publications For Anti-KIR5.1 KCNJ16 Antibody (A10858-1)
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Customer Q&As
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7 Customer Q&As for Anti-KIR5.1 KCNJ16 Antibody
Question
Is this A10858-1 anti-KIR5.1 antibody reactive to the isotypes of KCNJ16?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
The immunogen of A10858-1 anti-KIR5.1 antibody is Synthesized peptide derived from mouse KIR5.1 around the non-phosphorylation site of S416. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-10
Question
We are currently using anti-KIR5.1 antibody A10858-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
The anti-KIR5.1 antibody (A10858-1) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-29
Question
Would anti-KIR5.1 antibody A10858-1 work on pig WB with kidney parathyroid?
B. Moore
Verified customer
Asked: 2019-11-01
Answer
Our lab technicians have not tested anti-KIR5.1 antibody A10858-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-KIR5.1 antibody A10858-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig kidney parathyroid in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-01
Question
Would anti-KIR5.1 antibody A10858-1 work for WB with adult mammalian kidney?
J. Jones
Verified customer
Asked: 2019-10-16
Answer
According to the expression profile of adult mammalian kidney, KCNJ16 is highly expressed in adult mammalian kidney. So, it is likely that anti-KIR5.1 antibody A10858-1 will work for WB with adult mammalian kidney.
Boster Scientific Support
Answered: 2019-10-16
Question
I was wanting to use your anti-KIR5.1 antibody for WB for rat adult mammalian kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat adult mammalian kidney identification?
S. Mitchell
Verified customer
Asked: 2017-04-19
Answer
It shows on the product datasheet, A10858-1 anti-KIR5.1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat adult mammalian kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-04-19
Question
Does A10858-1 anti-KIR5.1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Wu
Verified customer
Asked: 2015-04-08
Answer
It shows on the product datasheet, A10858-1 anti-KIR5.1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-04-08
Question
Is a blocking peptide available for product anti-KIR5.1 antibody (A10858-1)?
A. Parker
Verified customer
Asked: 2013-02-25
Answer
We do provide the blocking peptide for product anti-KIR5.1 antibody (A10858-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2013-02-25
