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Product Info Summary
| SKU: | M14209 |
|---|---|
| Size: | 100 µg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-KRT77 Antibody (Monoclonal, ABM5G22)
SKU/Catalog Number
M14209
Size
100 µg
Form
Liquid
Description
Boster Bio Anti-KRT77 Antibody (Monoclonal, ABM5G22) (Catalog# M14209). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-KRT77 Antibody (Monoclonal, ABM5G22) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M14209)
Host
Mouse
Contents
100 µg in 200 µl PBS containing 0.05% stabilizing protein and 0.05% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABM5G22
Isotype
IgG2b Kappa
Immunogen
A partial length recombinant protein of KRT77 (amino acid 133-346)
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M14209 is reactive to KRT77 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
61.9 kDa
Background of KRT77
This monoclonal antibody recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. This monoclonal antibody recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. SPM115/SPM116 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M14209 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot: 2-4 µg/ml
Validation Images & Assay Conditions
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Expression analysis of KRT77. Anti-KRT77 antibody (Clone: ABM5G22) was tested at 2 &mu
Specific Publications For Anti-KRT77 Antibody (Monoclonal, ABM5G22) (M14209)
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