Anti-L1/ORF2 Antibody

Boster Bio Anti-L1/ORF2 Antibody (Catalog # A19762). Tested in Dot blot, ELISA, WB applications. This antibody reacts with Human, Chimpanzee.

Product Info Summary

SKU: A19762
Size: 100ug
Reactive Species: Human, Chimpanzee
Host: Chicken
Application: Dot blot, ELISA, WB

Product Name

Anti-L1/ORF2 Antibody

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SKU/Catalog Number

A19762

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-L1/ORF2 Antibody (Catalog # A19762). Tested in Dot blot, ELISA, WB applications. This antibody reacts with Human, Chimpanzee.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-L1/ORF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A19762)

Host

Chicken

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgY

Immunogen

This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH).  The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A19762 is reactive to in Human, Chimpanzee

Applications

A19762 is guaranteed for Dot blot, ELISA, WB Boster Guarantee

Observed Molecular Weight

19 kDa

Calculated molecular weight

23354 MW

Background of

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. The L1 (LINE-1), or Long INterspersed Element, retrotransposon ORF2 is the most common open reading frame in the human genome, present in various forms in many thousands of copies. This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signaling. Both intact ORF1 and ORF2 are absolutely required for autonomous retro-transposition. ORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2 appears to be tightly regulated except in germ line tissues, embryonic tissues, and certain cancers including teratomas, testicular cancers, and leukemias. This antibody is intended for use in studying control of L1 retrotransposons.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 2.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000
WB: 1:2,000-1_10,000

Validation Images & Assay Conditions

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6 Customer Q&As for Anti-L1/ORF2 Antibody

Question

Would A19762 anti-L1/ORF2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-08-08

Answer

It shows on the product datasheet, A19762 anti-L1/ORF2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-08-08

Question

We are currently using anti-L1/ORF2 antibody A19762 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-20

Answer

The anti-L1/ORF2 antibody (A19762) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-20

Question

Is a blocking peptide available for product anti-L1/ORF2 antibody (A19762)?

Verified Customer

Verified customer

Asked: 2019-05-14

Answer

We do provide the blocking peptide for product anti-L1/ORF2 antibody (A19762). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-05-14

Question

I see that the anti-L1/ORF2 antibody A19762 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-06-19

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-06-19

Question

My question regarding product A19762, anti-L1/ORF2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

R. Parker

Verified customer

Asked: 2017-01-06

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A19762 anti-L1/ORF2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-01-06

Question

Do you have a BSA free version of anti-L1/ORF2 antibody A19762 available?

S. Collins

Verified customer

Asked: 2016-02-03

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-L1/ORF2 antibody A19762 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2016-02-03

Order DetailsPrice
A19762

100 µg

$599

Free Secondary Antibody

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A19762
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$599.00

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