Product Info Summary
SKU: | M04320-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Monkey, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Thymidylate Synthase TYMS Monoclonal Antibody
View all Thymidylate Synthase Antibodies
SKU/Catalog Number
M04320-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Thymidylate Synthase TYMS Monoclonal Antibody catalog # M04320-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Thymidylate Synthase TYMS Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04320-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADAA-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Thymidylate Synthase Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M04320-1 is reactive to TYMS in Human, Monkey, Mouse, Rat
Applications
M04320-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
36 kDa
Calculated molecular weight
35.716kDa
Background of Thymidylate Synthase
Plays a major role in tight junction-specific obliteration of the intercellular space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TYMS using anti-TYMS antibody (M04320-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: rat thymus tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse thymus tissue lysates,
Lane 8: mouse SP2/0 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TYMS antigen affinity purified monoclonal antibody (Catalog # M04320-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TYMS at approximately 36 kDa. The expected band size for TYMS is at 36 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TYMS (Source: Uniprot.org, NCBI)
Gene Name
TYMS
Full Name
Thymidylate synthase
Weight
35.716kDa
Superfamily
thymidylate synthase family
Alternative Names
EC 2.1.1.45; HsT422; MGC88736; thymidylate synthase; thymidylate synthetase; TMSTsase; TSase; TSHST422 TYMS HST422, TMS, TS thymidylate synthetase thymidylate synthase|TSase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TYMS, check out the TYMS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TYMS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Thymidylate Synthase TYMS Monoclonal Antibody (M04320-1)
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No publications found for M04320-1
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Thymidylate Synthase TYMS Monoclonal Antibody
Question
We are currently using anti-Thymidylate Synthase Monoclonal antibody M04320-1 for mouse tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
The anti-Thymidylate Synthase Monoclonal antibody (M04320-1) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-04