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- Table of Contents
TYMS antibody (M04320-1) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Monkey, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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IHC analysis of TYMS using anti-TYMS antibody (M04320-1).
TYMS was detected in a paraffin-embedded section of human B lymphoblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TYMS Antibody (M04320-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
TYMS was detected in a paraffin-embedded section of human B lymphoblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TYMS Antibody (M04320-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Designing a Western blot or IHC for TYMS? The guides cover the expected band size and staining pattern, antibodies backed by real images, the controls to run, and protocols from published papers.
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Anti-Thymidylate Synthase TYMS Monoclonal Antibody
TYMS antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
TYMS antibody was positively detected by WB in:
human Hela whole cell, human Jurkat whole cell, human 293T whole cell, monkey COS-7 whole cell, rat thymus tissue, rat PC-12 whole cell, mouse thymus tissue, mouse SP2/0 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TYMS antibody was positively detected by IHC in:
human B lymphoblastoma tissue, human spleen tissue, human tonsil tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
IP
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Monkey
Tested
Recommend
Recommend
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-Thymidylate Synthase TYMS Monoclonal Antibody
View all TYMS Antibodies
View all TYMS Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADAA-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Thymidylate Synthase Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
36 kDa
Calculated Molecular Weight
35.7 kDa
Cite This Product
Anti-Thymidylate Synthase TYMS Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04320-1)
Protocols
Western Blot protocol for TYMS antibody
Immunohistochemistry (IHC) protocol for TYMS antibody
Publications
Application:
Species:
Exploring Protein Expression Profiles in Lung Cancer Insufficient Microwave Ablation: Implications for Recurrence
Published: 2025-04-02
Journal: OncoTargets and Therapy
Application: IHC
Impact Factor: 2.80
Species: Mouse
PMID: 40196869
Predictive value of thymidylate synthase for the prognosis and survival of lung adenocarcinoma patients
Published: 2014-11-03
Journal: Oncology Letters
Application: IHC
Impact Factor: 0.99
Species: Human
PMID: 25435969
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Product Promise
We promise Anti-Thymidylate Synthase TYMS Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M04320-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 26, 2026