Monkey primate Epiregulin ELISA Kit PicoKine®

Epiregulin ELISA kit for Monkey

Monkey primate Epiregulin ELISA Kit PicoKine™ (96 Tests). Quantitate Monkey EREG in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.5pg/ml.

Product Info Summary

SKU: EK1394-PR
Size: 96 wells/kit, with removable strips.
Reactive Species: Monkey
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Monkey primate Epiregulin ELISA Kit PicoKine®

View all Epiregulin ELISA kits

SKU/Catalog Number

EK1394-PR

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Monkey primate Epiregulin ELISA Kit PicoKine™ (96 Tests). Quantitate Monkey EREG in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.5pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Monkey primate Epiregulin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1394-PR)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V60-L108

Sensitivity

<10 pg/ml

Assay Range

3.9 pg/ml - 250 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1394-PR for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1394-PR is reactive to EREG in Monkey samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK1394-PR is guaranteed for ELISA in Monkey by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Epiregulin

Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.

Kit Components

Catalog Number Description Quantity
EK1394-PR-CAP Anti-Monkey EREG Pre-coated 96-well strip microplate 1
EK1394-PR-ST Monkey EREG Standard 2 vials, 10 ng/tube
EK1394-PR-DA Monkey EREG Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03.97.815.631.262.5125250
O.D.0.0630.1030.1800.3010.5310.87791.4262.062

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Monkey primate Epiregulin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)26.288.714425.491.5151
Standard deviation1.187.946.811.737.0511.93
CV (%)4.55.85.76.87.77.9

Gene/Protein Information For EREG (Source: Uniprot.Org, NCBI)

Gene Name

EREG

Full Name

Proepiregulin

Weight

19.044kDa

Alternative Names

Epiregulin; ER; EREG; proepiregulin EREG EPR, ER, Ep epiregulin proepiregulin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on EREG, check out the EREG Infographic

EREG infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for EREG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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6 Customer Q&As for Monkey primate Epiregulin ELISA Kit PicoKine®

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-01-09

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-01-09

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

L. Huang

Verified customer

Asked: 2019-11-16

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-11-16

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Z. Taylor

Verified customer

Asked: 2018-06-05

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-06-05

Question

Q: how to analyze ELISA data? I have performed Epiregulin level in plasma.

W. Evans

Verified customer

Asked: 2018-01-09

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-01-09

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Epiregulin using the EK1394-PR Monkey Epiregulin Picokine® ELISA Kit?

P. Scott

Verified customer

Asked: 2017-05-16

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2017-05-16

Question

Q: can I use citrate plasma as samples in Monkey Epiregulin Picokine® ELISA Kit (Catalog # EK1394-PR)?

Verified Customer

Verified customer

Asked: 2016-10-11

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Epiregulin causing disruption of its protein structure. Epiregulin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Epiregulin ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2016-10-11

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