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MAP1LC3B antibody (M01524) performs well in IP, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 44 Publications

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Immunofluorescent analysis using the Antibody at 1:150 dilution.

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Anti-LC3B MAP1LC3B Rabbit Monoclonal Antibody

Name: Anti-LC3B MAP1LC3B Rabbit Monoclonal Antibody
Brand: Boster Bio
SKU: M01524
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

MAP1LC3B antibody

44 Publications|

1 Review

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Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	IP, IF, ICC, WB

Tested Applications

MAP1LC3B antibody was positively detected by WB in:

human Hela whole cell, human MCF-7 whole cell, human A549 whole cell, human U2OS whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neurao-2a whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

MAP1LC3B antibody was positively detected by IHC in:

human glioma tissue, mouse brain tissue, rat brain tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

MAP1LC3B antibody was positively detected by ICC/IF in:

Hela cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Product Information

Product Name

Anti-LC3B MAP1LC3B Rabbit Monoclonal Antibody
View all MAP1LC3B Antibodies

Form

Liquid

Uniprot

Q9GZQ8

Gene ID

81631

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

IEC-13

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human LC3B

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

15, 18 kDa

Calculated Molecular Weight

14.7 kDa

Cite This Product

Anti-LC3B MAP1LC3B Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01524)

Protocols

Western Blot protocol for MAP1LC3B antibody

Review Protocol

Immunohistochemistry (IHC) protocol for MAP1LC3B antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for MAP1LC3B antibody

Review Protocol

Publications

Salidroside alleviates sepsis-associated acute lung injury through modulation of the ERK/FoxO3a signaling axis and improvement of impaired autophagic flux

Published: 2026-08-24

Journal: MOLECULAR BIOLOGY REPORTS

Application: WB

Impact Factor: 3.20

Species: Rat

PMID: 42635681

SKU	M01524
Application	Western Blot
Sample	Human HepG2 cells
Sample Processing Description	Total proteins were extracted from normally cultured HepG2 cells.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody	LC3B MAP1LC3B Rabbit Monoclonal Antibody
Primary Incubation	1:5000, overnight at 4 ℃
Secondary Antibody	HRP-conjugated goat anti-rabbit IgG
Secondary Incubation	1:10000, 1 hour in RT
Detection	Substrate: ECL substrate; Image system: ChemiDoc MP
Results Summary	LC3B is a widely studied clinical biomarker and therapeutic target regulated by neddylation modification, and is a recognized gold-standard marker for dynamic monitoring of autophagy activity. In this experiment, normally cultured HepG2 cells without any drug treatment were used to validate the LC3B antibody. The results showed clear target bands, including the higher molecular weight LC3B-I band and the lower molecular weight LC3B-II band, with clean background, demonstrating excellent antibody performance.

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Anti-LC3B MAP1LC3B Rabbit Monoclonal Antibody

MAP1LC3B antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Salidroside alleviates sepsis-associated acute lung injury through modulation of the ERK/FoxO3a signaling axis and improvement of impaired autophagic flux

Trichostatin A improves impaired autophagic flux associated with ERK/FoxO3a signaling to alleviate acute pancreatitis

Urolithin C Exerts Anti-Endometrial Cancer Effects by Inducing Autophagy Through Specific Stimulation of ATF3

ACE2 ameliorates DOX-induced cardiotoxicity by suppressing excessive autophagy via the AMPK/mTOR signaling pathway

Echinacoside improves antioxidant responses and angiogenesis through Parkin-MFN2-mediated mitophagy to promote diabetic wound healing

Reactive oxygen species-mediated epidermal stress in human keratinocytes under dual exposure to blue light and heated tobacco product

Gymconopin C exhibits anti-non-small cell lung cancer effect by regulating miR-6777-5p/ADRB2 pathway to promote mitophagy

Songorine inhibits mitophagy in chronic heart failure via the TBC1D15/Fis1/Rab7A pathway

Forsythiaside A ameliorates acetaminophen-induced liver injury by suppressing ferroptosis through activating USP11/SIRT3-mediated mitophagy

Fibroblast growth factor 21 responds to taurocholic acid sodium-induced acute pancreatic injury

Synergistic effects of notoginsenoside R1 and saikosaponin B2 in atherosclerosis: A novel approach targeting PI3K/AKT/mTOR pathway and macrophage autophagy

Diosgenin alleviates neuropathic pain based on the enhancement of autophagy and promotion of M2 polarization

Hypoxia-induced autophagy attenuates ferredoxin 1-mediated cuproptosis in colorectal cancer cells

Aconiti Lateralis Radix Praeparata ameliorates heart failure via PI3K/AKT/Bnip3 pathway

Resveratrol alleviates testicular toxicity induced by anti-PD-1 through regulating the NRF2-SLC7A11-GPX4 pathway

Knockdown of NDUFAF6 inhibits breast cancer progression via promoting mitophagy and apoptosis

Maduramicin ammonium impairs autophagic flux through activating AMPK-mediated eIF2α-ATF4 endoplasmic reticulum stress pathway in skeletal muscle

Carvacrol acetate activated Nrf2 modulates mitophagy for the treatment of neurocyte oxidative stress induced by chlorpyrifos

The Therapeutic Mechanisms of Shenyan Oral Liquid I Against Chronic Kidney Disease Based on Network Pharmacology and Experimental Validation

TMEM232 is required for the formation of sperm flagellum and male fertility in mice

Irisin alleviates the pyroptosis of β cells in T2DM by inhibiting NLRP3 inflammasome through regulating miR-19b-3p/SOCS3/STAT3 axis mediated autophagy

Mitoxantrone ameliorates ineffective erythropoiesis in a β-thalassemia intermedia mouse model

VD3/VDR attenuates bisphenol A–induced impairment in mouse Leydig cells via regulation of autophagy

Ruthenium Red Alleviates Post-Resuscitation Myocardial Dysfunction by Upregulating Mitophagy through Inhibition of USP33 in a Cardiac Arrest Rat Model

Liensinine sensitizes colorectal cancer cells to oxaliplatin by targeting HIF-1a to inhibit autophagy

STAM2 negatively regulates the MyD88-mediated NF-κB signaling pathway in miiuy croaker, Miichthys miiuy

m6A-methylated KCTD21-AS1 regulates macrophage phagocytosis through CD47 and cell autophagy through TIPR

Activation of endoplasmic reticulum stress by harmine suppresses the growth of esophageal squamous cell carcinoma

Lower dose of metformin combined with artesunate induced autophagy-dependent apoptosis of glioblastoma by activating ROS-AMPK-mTOR axis

The mtDNA-STING pathway plays an important role in both navitoclax- and S63845-induced autophagy and enhances cell death

Multibiofunctional TFEB-engineered endothelial progenitor cell-derived extracellular vesicles/hydrogel system for rescuing critical limb ischemia

ACKR4a induces autophagy to block NF-κB signaling and apoptosis to facilitate Vibrio harveyi infection

Cordycepin alleviated metabolic inflammation in Western diet-fed mice by targeting intestinal barrier integrity and intestinal flora

eIF3k inhibits NF-κB signaling by targeting MyD88 for ATG5-mediated autophagic degradation in teleost fish

Cu-Doped-ZnO Nanocrystals Induce Hepatocyte Autophagy by Oxidative Stress Pathway

Rhodiola crenulata extract decreases fatty acid oxidation and autophagy to ameliorate pulmonary arterial hypertension by targeting inhibition of acylcarnitine in rats

Selective binding of mitophagy receptor protein Bcl-rambo to LC3/GABARAP family proteins

Exogenous Hydrogen Sulfide Ameliorates Diabetic Myocardial Fibrosis by Inhibiting Cell Aging Through SIRT6/AMPK Autophagy

β-Cypermethrin promotes the adipogenesis of 3T3-L1 cells via inducing autophagy and shaping an adipogenesis-friendly microenvironment

Huganpian, a traditional chinese medicine, inhibits liver cancer growth in vitro and in vivo by inducing autophagy and cell cycle arrest

Hydroxytyrosol prevents dermal papilla cells inflammation under oxidative stress by inducing autophagy

Autophagy protects murine macrophages from β-cypermethrin-induced mitochondrial dysfunction and cytotoxicity via the reduction of oxidation stress

Expression of miR-551b and its effect on apoptosis in human gastric carcinoma

Antagomirs Targeting MiroRNA-134 Attenuates Epilepsy in Rats through Regulation of Oxidative Stress, Mitochondrial Functions and Autophagy

Review

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1 Review For Anti-LC3B MAP1LC3B Rabbit Monoclonal Antibody

LC3B antibody (M01524) was validated by WB using human HepG2 cells, showing clear LC3B-I and LC3B-II bands with clean background and reliable performance.

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