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Product Info Summary
| SKU: | A00350 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC |
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Product info
Product Name
Anti-Leptin Receptor/LEPR Antibody
SKU/Catalog Number
A00350
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Leptin Receptor/LEPR Antibody catalog # A00350. Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Leptin Receptor/LEPR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00350)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Leptin Receptor, different from the related mouse sequence by nine amino acids, and from the related rat sequence by eight amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A00350 is reactive to LEPR in Human, Mouse, Rat
Calculated molecular weight
132.5 kDa
Background of LEPR
Leptin receptor, also known as LEP-R or OB-R is a protein that in humans is encoded by the LEPR gene. The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00350 is guaranteed for ELISA, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
IHC: mouse kidney tissue, rat lung tissue
Validation Images & Assay Conditions
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IHC analysis of Leptin Receptor/LEPR using anti-Leptin Receptor/LEPR antibody (A00350).
Leptin Receptor/LEPR was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Leptin Receptor/LEPR Antibody (A00350) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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IHC analysis of LEPR using anti-LEPR antibody (A00350).
LEPR was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LEPR Antibody (A00350) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Effects of long-term L -serine administration on the leptin signaling pathway in aging mice. (A–E) LepRb expression as determined by immunohistochemistry; (F) leptin content in the hypothalamus; (G) relative LepRb mRNA expression; (H,I) relative pSTAT3 protein expression. Aged, untreated control mice; Low-ser, mice fed with 0.1% (wt/vol) L -serine dissolved in the drinking water; Middle-ser, mice fed with 0.2% (wt/vol) L -serine dissolved in the drinking water; High-ser, mice fed with 0.5% (wt/vol) L -serine dissolved in the drinking water. Young, adult male mice at the age of 18 months. Arrow (yellow), expression of LepRb protein; LepRb, leptin receptor. Values are expressed as mean ± SEM, n = 3 for the statistical analysis of western blotting data and n = 8 for the statistical analysis of other data; a, b Means of the bars with different letters were significantly different among groups ( p < 0.05).
Index in PubMed under a CC BY license. PMID: 30190704
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IHC analysis of Leptin Receptor/LEPR using anti-Leptin Receptor/LEPR antibody (A00350).
Leptin Receptor/LEPR was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Leptin Receptor/LEPR Antibody (A00350) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-Leptin Receptor/LEPR Antibody (A00350)
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1 Customer Q&As for Anti-Leptin Receptor/LEPR Antibody
Question
We are currently using anti-Leptin Receptor/LEPR antibody A00350 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
The anti-Leptin Receptor/LEPR antibody (A00350) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-22
