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- Table of Contents
LEPR antibody (A00350) performs well in ELISA, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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IHC analysis of LEPR using anti-LEPR antibody (A00350).
LEPR was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LEPR Antibody (A00350) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
LEPR was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LEPR Antibody (A00350) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Leptin Receptor/LEPR Antibody
LEPR antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IHC, WB |
Tested Applications
LEPR antibody was positively detected by WB in:
human K562 whole cell, human HeLa whole cell, human HepG2 whole cell, human HEL whole cell, rat lung tissue, rat kidney tissue, mouse lung tissue, mouse kidney tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
LEPR antibody was positively detected by IHC in:
human liver tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Dog
Predicted
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Leptin Receptor/LEPR Antibody
View all LEPR Antibodies
View all LEPR Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Leptin Receptor, different from the related mouse sequence by nine amino acids, and from the related rat sequence by eight amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
150 kDa
Calculated Molecular Weight
132.5 kDa
Background of LEPR
Leptin receptor, also known as LEP-R or OB-R is a protein that in humans is encoded by the LEPR gene. The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Cite This Product
Anti-Leptin Receptor/LEPR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00350)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Dog
94%
High Homology
Monkey
94%
High Homology
Sheep
89%
Moderate Homology
Rabbit
87%
Moderate Homology
Cow
87%
Moderate Homology
Rat
83%
Moderate Homology
Mouse
81%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for LEPR antibody
Immunohistochemistry (IHC) protocol for LEPR antibody
Publications
Application:
Species:
Leptin decreases the transcription of BKCa channels and Gs to Gi protein-ratio in late pregnant rat uterus
Published: 2023-09-26
Journal: GENE
Application: WB
Impact Factor: 3.50
Species: Rat
PMID: 37769981
Long-Term l-Serine Administration Reduces Food Intake and Improves Oxidative Stress and Sirt1/NFκB Signaling in the Hypothalamus of Aging Mice
Published: 2018-08-23
Journal: Frontiers in Endocrinology
Application: IHC
Impact Factor: 3.52
Species: Mouse
PMID: 30190704
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We promise Anti-Leptin Receptor/LEPR Antibody performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A00350
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026