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Product Info Summary
| SKU: | M01323 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-LIM Kinase 1 (LIMK1) Mouse Monoclonal Antibody [Clone ID: OTI6B4]
SKU/Catalog Number
M01323
Size
100 µl
Description
Boster Bio LIMK1 (LIM Kinase 1 ) mouse monoclonal antibody, clone OTI6B4 (formerly 6B4). Catalog# M01323. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-LIM Kinase 1 (LIMK1) Mouse Monoclonal Antibody [Clone ID: OTI6B4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01323)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI6B4
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human LIMK1 (NP_002305) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01323 is reactive to LIMK1 in Dog, Human, Monkey, Mouse, Rat
Reconstitution
Calculated molecular weight
72.585kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01323 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIMK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIMK1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LIMK1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either LIMK1 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIMK1 antibody (M01323)
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Anti-LIMK1 mouse monoclonal antibody (M01323) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIMK1.
Protein Target Info & Infographic
Gene/Protein Information For LIMK1 (Source: Uniprot.org, NCBI)
Gene Name
LIMK1
Full Name
LIM domain kinase 1
Weight
72.585kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11.1; LIM domain kinase 1; LIM motif-containing protein kinase; LIMKLIMK-1 LIMK1 LIMK, LIMK-1 LIM domain kinase 1 LIM domain kinase 1|LIM motif-containing protein kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LIMK1, check out the LIMK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LIMK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LIM Kinase 1 (LIMK1) Mouse Monoclonal Antibody [Clone ID: OTI6B4] (M01323)
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