Product Info Summary
| SKU: | RP1081 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Livin/BIRC7 Antibody Picoband®
SKU/Catalog Number
RP1081
PB0645 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Livin/BIRC7 Antibody catalog # RP1081. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Livin/BIRC7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1081)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Livin recombinant protein (Position: E87-S298). Human Livin shares 69.8% amino acid (aa) sequence identity with mouse Livin.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1081 is reactive to BIRC7 in Human
Observed Molecular Weight
33 kDa
Calculated molecular weight
32.8 kDa
Background of BIRC7
Baculoviral IAP repeat-containing protein 7 is a protein that in humans is encoded by the BIRC7 gene. This protein is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) and a RING finger motif. Its gene is mapped to 20q13.3. It has got 280- and 298- amino acids. The protein is highly expressed in melanomas while lowly expressed in some lymphomas, fetal kidney, fetal liver, testis and thymus. BIRC7 plays a vital role in blocking apoptosis induced.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1081 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, -
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: HELA Whole Cell, A431 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-Livin antibody, RP1081, Western blotting
All lanes: Anti Livin (RP1081) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A431 Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 33KD
Specific Publications For Anti-Livin/BIRC7 Antibody Picoband® (RP1081)
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Customer Q&As
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7 Customer Q&As for Anti-Livin/BIRC7 Antibody Picoband®
Question
We are currently using anti-Livin/BIRC7 antibody RP1081 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
The anti-Livin/BIRC7 antibody (RP1081) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-28
Question
I was wanting to use your anti-Livin/BIRC7 antibody for ELISA for human skin on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human skin identification?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
As indicated on the product datasheet, RP1081 anti-Livin/BIRC7 antibody has been validated for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-29
Question
Will anti-Livin/BIRC7 antibody RP1081 work for ELISA with skin?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
According to the expression profile of skin, BIRC7 is highly expressed in skin. So, it is likely that anti-Livin/BIRC7 antibody RP1081 will work for ELISA with skin.
Boster Scientific Support
Answered: 2019-06-10
Question
Is a blocking peptide available for product anti-Livin/BIRC7 antibody (RP1081)?
Verified Customer
Verified customer
Asked: 2019-01-02
Answer
We do provide the blocking peptide for product anti-Livin/BIRC7 antibody (RP1081). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-01-02
Question
I am looking for to test anti-Livin/BIRC7 antibody RP1081 on human skin for research purposes, then I may be interested in using anti-Livin/BIRC7 antibody RP1081 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Williams
Verified customer
Asked: 2018-06-26
Answer
The products we sell, including anti-Livin/BIRC7 antibody RP1081, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-06-26
Question
Is this RP1081 anti-Livin/BIRC7 antibody reactive to the isotypes of BIRC7?
Verified Customer
Verified customer
Asked: 2017-10-23
Answer
The immunogen of RP1081 anti-Livin/BIRC7 antibody is E. coli-derived human Livin recombinant protein (Position: E87-S298). Human Livin shares 69.8% amino acid (aa) sequence identity with mouse Livin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-23
Question
Is there a BSA free version of anti-Livin/BIRC7 antibody RP1081 available?
R. Jones
Verified customer
Asked: 2016-07-21
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Livin/BIRC7 antibody RP1081 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-07-21


