Anti-LC3B/MAP1LC3B Antibody

Western blot analysis of LC3B/MAP1LC3B using anti-LC3B/MAP1LC3B antibody (A01524-2).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U2OS whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LC3B/MAP1LC3B antigen affinity purified polyclonal antibody (A01524-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LC3B/MAP1LC3B at approximately 15,18 kDa. The expected band size for LC3B/MAP1LC3B is at 15 kDa.

IHC analysis of LC3B/MAP1LC3B using anti-LC3B/MAP1LC3B antibody (A01524-2).
LC3B/MAP1LC3B was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-LC3B/MAP1LC3B Antibody (A01524-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of LC3B/MAP1LC3B using anti-LC3B/MAP1LC3B antibody (A01524-2).
LC3B/MAP1LC3B was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-LC3B/MAP1LC3B Antibody (A01524-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of LC3B/MAP1LC3B using anti-LC3B/MAP1LC3B antibody (A01524-2).
LC3B/MAP1LC3B was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-LC3B/MAP1LC3B Antibody (A01524-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

PBM alleviates abnormal mitochondrial autophagy and promotes mitochondrial energy metabolism in mice with AD. A RT-PCR was used to detect autophagy-related protein (Beclin1, LC3II.) and glycolysis-related proteins (TSPO and HK2) in mouse brain tissues. B WB was used to detect the autophagy protein LC3II, and the oxidative phosphorylation-related proteins PGC-1α and NRF-1. C Immunohistochemical staining of mouse brains using an anti-HK2 antibody (scale bars 100 μm and 20 μm, respectively) with brown plaques of HK2. D , E WB was used to detect the glycolysis-related proteins GLUT1, PKM2, and HK2. Experimental data are presented as the mean ± standard deviation. Compared with the CON group, * p < 0.05, ** p < 0.01, *** p < 0.001; Compared with the AD group, # p < 0.05, ## p < 0.01
Index in PubMed under a CC BY license. PMID: 40188044