Anti-MED18 Antibody Picoband™

MED18 antibody

Boster Bio Anti-MED18 Antibody Picoband™ catalog # A10600-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A10600-2
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-MED18 Antibody Picoband™

View all MED18 Antibodies

SKU/Catalog Number

A10600-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MED18 Antibody Picoband™ catalog # A10600-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MED18 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10600-2)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human MED18 recombinant protein (Position: M1-M208).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A10600-2 is reactive to MED18 in Human, Mouse, Rat

Applications

A10600-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

24 kDa

Calculated molecular weight

23.663kDa

Background of MED18

MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II. Using in vitro-translated epitope-tagged proteins for protein-binding assays, it is found that MED18 directly interacted with the Mediator subunit TRFP (MED20). The MED18-TRFP heterodimer could also be coimmunoprecipitated from cotransfected insect cells. The MED18 gene was mapped to chromosome 1p35.3 based on an alignment of the MED18 sequence with the genomic sequence.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For MED18 (Source: Uniprot.org, NCBI)

Gene Name

MED18

Full Name

Mediator of RNA polymerase II transcription subunit 18

Weight

23.663kDa

Superfamily

Mediator complex subunit 18 family

Alternative Names

FLJ20045; mediator complex subunit 18mediator of RNA polymerase II transcription subunit 18; mediator of RNA polymerase II transcription, subunit 18 homolog (S. cerevisiae); p28bmediator of RNA polymerase II transcription, subunit 18 homolog; TRAP/mediator complex subunit p28b MED18 SRB5, p28b mediator complex subunit 18 mediator of RNA polymerase II transcription subunit 18|TRAP/mediator complex subunit p28b|mediator of RNA polymerase II transcription, subunit 18 homolog

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MED18, check out the MED18 Infographic

MED18 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MED18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-MED18 Antibody Picoband™

Question

We are currently using anti-MED18 antibody A10600-2 for mouse tissue, and we are content with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?

S. Huang

Verified customer

Asked: 2014-04-21

Answer

The anti-MED18 antibody (A10600-2) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-04-21

Order DetailsPrice
A10600-2

100μg

$370
A10600-2-10ug

10μg sample (liquid)

$99
A10600-2-Biotin

100 μg Biotin conjugated

$570
A10600-2-Cy3

100 μg Cy3 conjugated

$570
A10600-2-Dylight488

100 μg Dylight488 conjugated

$570
A10600-2-Dylight550

100 μg Dylight550 conjugated

$570
A10600-2-Dylight594

100 μg Dylight594 conjugated

$570
A10600-2-FITC

100 μg FITC conjugated

$570
A10600-2-HRP

100 μg HRP conjugated

$570
A10600-2-APC

100 μg APC conjugated

$670
A10600-2-PE

100 μg PE conjugated

$670
A10600-2-carrier-free

Carrier Free

$370

More conjugates/formats

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A10600-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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