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Product Info Summary
| SKU: | M00489 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-MERTK/Mer Rabbit Monoclonal Antibody
SKU/Catalog Number
M00489
BM4167 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MERTK/Mer Rabbit Monoclonal Antibody catalog # M00489. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MERTK/Mer Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00489)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
COI-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MERTK
Reactive Species
M00489 is reactive to MERTK in Human
Observed Molecular Weight
188 kDa
Calculated molecular weight
110.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00489 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:20
Positive Control
WB: HEK293 cell
IHC: human lymphoma tissue
Validation Images & Assay Conditions
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Western blot analysis of MERTK expression in HEK293 cell lysates.
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Immunohistochemical analysis of paraffin-embedded human lymphoma, using MERTK Antibody.
Specific Publications For Anti-MERTK/Mer Rabbit Monoclonal Antibody (M00489)
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Customer Q&As
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4 Customer Q&As for Anti-MERTK/Mer Rabbit Monoclonal Antibody
Question
We are currently using anti-MERTK/Mer Rabbit Monoclonal antibody M00489 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
The anti-MERTK/Mer Rabbit Monoclonal antibody (M00489) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-20
Question
We have tried in the past anti-MERTK/Mer Rabbit Monoclonal antibody for WB on right adrenal gland cortex last year. I am using human, and I plan to use the antibody for IHC next. I was wanting to use examining right adrenal gland cortex as well as plasma in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2018-01-05
Answer
I have checked the website and datasheets of our anti-MERTK/Mer Rabbit Monoclonal antibody and I see that M00489 has been validated on human in both WB and IHC. Thus M00489 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-01-05
Question
My colleagues were happy with the WB result of your anti-MERTK/Mer Rabbit Monoclonal antibody. However we have been able to see positive staining in right adrenal gland cortex membrane using this antibody. Is that expected? Could you tell me where is MERTK supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-07-06
Answer
According to literature, right adrenal gland cortex does express MERTK. Generally MERTK expresses in membrane. Regarding which tissues have MERTK expression, here are a few articles citing expression in various tissues:
Peripheral blood leukocyte, Pubmed ID: 8086340, 11062461
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2017-07-06
Question
We have seen staining in human plasma. Any tips? Is anti-MERTK/Mer Rabbit Monoclonal antibody supposed to stain plasma positively?
C. Lewis
Verified customer
Asked: 2014-10-29
Answer
From literature plasma does express MERTK. From Uniprot.org, MERTK is expressed in right adrenal gland cortex, peripheral blood leukocyte, plasma, among other tissues. Regarding which tissues have MERTK expression, here are a few articles citing expression in various tissues:
Peripheral blood leukocyte, Pubmed ID: 8086340, 11062461
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2014-10-29
