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Product Info Summary
| SKU: | A30776 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-MOX2R CD200R1 Antibody
SKU/Catalog Number
A30776
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MOX2R CD200R1 Antibody (Catalog# A30776). Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MOX2R CD200R1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30776)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human MOX2R. Immunogen sequence location: 241 - 290
Reactive Species
A30776 is reactive to CD200R1 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
36.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30776 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
ELISA: 1:40000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western Blot analysis of HepG2 cells using OX2R Polyclonal Antibody
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Western Blot analysis of K562 cells using OX2R Polyclonal Antibody
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Western blot analysis of lysates from K562 cells, using MOX2R Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-MOX2R CD200R1 Antibody (A30776)
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Customer Q&As
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5 Customer Q&As for Anti-MOX2R CD200R1 Antibody
Question
We are currently using anti-MOX2R antibody A30776 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
The anti-MOX2R antibody (A30776) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-16
Question
Can you help my question with product A30776, anti-MOX2R antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30776 anti-MOX2R antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-03
Question
See attached the WB image, lot number and protocol we used for liver using anti-MOX2R antibody A30776. Please let me know if you require anything else.
S. Thomas
Verified customer
Asked: 2019-11-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-21
Question
Is there a BSA free version of anti-MOX2R antibody A30776 available?
Verified Customer
Verified customer
Asked: 2018-04-02
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MOX2R antibody A30776 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-04-02
Question
Will A30776 anti-MOX2R antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Thomas
Verified customer
Asked: 2016-08-18
Answer
You can see on the product datasheet, A30776 anti-MOX2R antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-08-18
