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Product Info Summary
| SKU: | A17659 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-MRGE MRGPRE Antibody
SKU/Catalog Number
A17659
Size
100μl
Form
Liquid
Description
Boster Bio Anti-MRGE MRGPRE Antibody catalog # A17659. Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRGE MRGPRE Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17659)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human MRGRE. AA range:171-220
Reactive Species
A17659 is reactive to MRGPRE in Human, Mouse, Rat
Calculated molecular weight
34.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A17659 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 1:2000-20000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using MRGE Polyclonal antibody.
Specific Publications For Anti-MRGE MRGPRE Antibody (A17659)
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5 Customer Q&As for Anti-MRGE MRGPRE Antibody
Question
My question regarding product A17659, anti-MRGE antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Anderson
Verified customer
Asked: 2020-03-10
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A17659 anti-MRGE antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-10
Question
Does A17659 anti-MRGE antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
As indicated on the product datasheet, A17659 anti-MRGE antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-03
Question
Does anti-MRGE antibody A17659 work for WB with ectocervix?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
According to the expression profile of ectocervix, MRGPRE is highly expressed in ectocervix. So, it is likely that anti-MRGE antibody A17659 will work for WB with ectocervix.
Boster Scientific Support
Answered: 2019-10-16
Question
I am interested in to test anti-MRGE antibody A17659 on human ectocervix for research purposes, then I may be interested in using anti-MRGE antibody A17659 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
The products we sell, including anti-MRGE antibody A17659, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-15
Question
I was wanting to use your anti-MRGE antibody for WB for human ectocervix on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human ectocervix identification?
R. Johnson
Verified customer
Asked: 2013-02-06
Answer
You can see on the product datasheet, A17659 anti-MRGE antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human ectocervix in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-02-06
