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Product Info Summary
| SKU: | A10693 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-MRP-L10 Antibody
SKU/Catalog Number
A10693
Size
100μl
Form
Liquid
Description
Boster Bio Anti-MRP-L10 Antibody catalog # A10693. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRP-L10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10693)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human MRPL10. AA range:211-260
Reactive Species
A10693 is reactive to MRPL10 in Human, Mouse, Rat
Calculated molecular weight
29.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10693 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using MRP-L10 Polyclonal Antibody diluted at 1:1000
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Western blot analysis of lysates from 293, HepG2, and COLO cells, using MRPL10 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-MRP-L10 Antibody (A10693)
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Customer Q&As
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5 Customer Q&As for Anti-MRP-L10 Antibody
Question
We are currently using anti-MRP-L10 antibody A10693 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
The anti-MRP-L10 antibody (A10693) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-06
Question
Does anti-MRP-L10 antibody A10693 work on pig WB with pancreas skin?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
Our lab technicians have not validated anti-MRP-L10 antibody A10693 on pig. You can run a BLAST between pig and the immunogen sequence of anti-MRP-L10 antibody A10693 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig pancreas skin in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-26
Question
Here is the WB image, lot number and protocol we used for caudate nucleus using anti-MRP-L10 antibody A10693. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-27
Question
Is this A10693 anti-MRP-L10 antibody reactive to the isotypes of MRPL10?
N. Rodriguez
Verified customer
Asked: 2015-09-04
Answer
The immunogen of A10693 anti-MRP-L10 antibody is Synthesized peptide derived from the C-terminal region of human MRP-L10. at AA rangle: 180-260. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-09-04
Question
I am interested in to test anti-MRP-L10 antibody A10693 on human caudate nucleus for research purposes, then I may be interested in using anti-MRP-L10 antibody A10693 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Krishna
Verified customer
Asked: 2013-08-23
Answer
The products we sell, including anti-MRP-L10 antibody A10693, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-08-23
