Rabbit IgG polyclonal antibody for Cytochrome c oxidase subunit 1(MT-CO1) detection. Tested with WB, IHC-P, ICC in Human.
Anti-MTCO1/MT-CO1 Antibody Info At A Glance
|Applications:||IHC, ICC, WB|
|Product Name||Anti-MTCO1/MT-CO1 Antibody
See all MT-CO1 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for MTCO1/MT-CO1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. MTCO1/MT-CO1 information: Molecular Weight: 57041 MW; Subcellular Localization: Mitochondrion inner membrane; Multi-pass membrane protein.|
|Cite This Product||Anti-MTCO1/MT-CO1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1317-1)|
|Applications||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human MTCO1(501-514aa PYHTFEEPVYMKS).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For MT-CO1 (Source: Uniprot.org, NCBI)
|Species Of This Entry||Human|
|Protein Name||Cytochrome c oxidase subunit 1|
|Superfamily||heme-copper respiratory oxidase family|
|Alternative Names||MT-CO1|Cytochrome c oxidase subunit 1|
|Subcellular Localization||Mitochondrion inner membrane; Multi-pass membrane protein.|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For MT-CO1 (Source: Uniprot.org, NCBI)
|Protein Function||Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1- 3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.|
|Interacting Proteins||COA3; COX4I1; TIMM21|
|Related Diseases||Leber hereditary optic neuropathy (LHON)|
|Background||Cytochrome c oxidase subunit I(CO1 or MTCO1) is 1 of 3 mitochondrial DNA(mtDNA) encoded subunits(MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides. Subunits I, II, and III(MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear encoded. The cytochrome c oxidase family of enzymes have 4 redox centers, 2 hemes and 2 copper centers. In mitochondrial Complex IV, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB. The 2 hemes and CuB are bound to subunit I. Acin-Perez et al.(2003) identified a cell line containing single and double missense mutations in the cytochrome c oxidase(COX) subunit I gene of mouse mitochondrial DNA. And they hypothesized that deleterious mutations can arise and become predominant; cultured cells can maintain several mtDNA haplotypes at stable frequencies; the respiratory chain has little spare COX capacity; and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme.|
|Scientific References||PMID: 7219534 by Anderson S., et al. Sequence and organization of the human mitochondrial genome.|
PMID: 7530363 by Horai S., et al. Recent African origin of modern humans revealed by complete sequences of hominoid mitochondrial DNAs.
Our Boster Quality Guarantee for Anti-MTCO1/MT-CO1 Antibody covers its use in the following applications.Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
MTCO1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTCO1 Antibody (PA1317-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
MTCO1 was detected in immunocytochemical section of C6 Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-MTCO1 Antibody (PA1317-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The samples were loaded under reducing conditions.
Lane 1: human HeLa mitochondria lysates at 20ug,
Lane 2: human HeLa whole cell lysates at 20ug,
Lane 3: human Caco-2 whole cell lysates at 50ug,
Lane 4: rat heart tissue lysates at 50ug.
Lane 5: mouse heart tissue lysates at 50ug.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 60 minutes. Blocked the membrane with 5% Non-fat Milk in TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- MTCO1 antigen affinity purified polyclonal antibody (Catalog # PA1317-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTCO1 at approximately 37KD. The expected band size for MTCO1 is at 57KD.
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