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Product Info Summary
| SKU: | PB9145 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-MUC1 Antibody
SKU/Catalog Number
PB9145
PB0155 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MUC1 Antibody catalog # PB9145. Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MUC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9145)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MUC1 recombinant protein (Position: R945-G1097). Human MUC1 shares 56% amino acid (aa) sequence identity with mouse MUC1.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9145 is reactive to MUC1 in Human
Calculated molecular weight
122.1 kDa
Background of MUC1
Mucin 1, cell surface associated (MUC1) or polymorphic epithelial mucin (PEM) is a mucin encoded by the MUC1 gene in humans. This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. It is mapped to 1q22. Mucin 1 is a transmembrane mucin normally expressed on the apical borders of secretory epithelial cells. Overexpression of Mucin 1 is often associated with colon, breast, ovarian, lung and pancreatic cancers. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Mucin 1 stimulated ESR1-mediated transcription and contributed to estradiol-mediated growth and survival of breast cancer cells. This gene also can suppress pulmonary innate immunity, and its antiinflammatory activity may play an important modulatory role during microbial infection.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9145 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Positive Control
IHC: human ovary cancer tissue
Validation Images & Assay Conditions
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IHC analysis of MUC1 using anti-MUC1 antibody (PB9145).
MUC1 was detected in a paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MUC1 Antibody (PB9145) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-MUC1 Antibody (PB9145)
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4 Customer Q&As for Anti-MUC1 Antibody
Question
My colleagues were content with the WB result of your anti-MUC1 antibody. However we have seen positive staining in mammary carcinoma isoform 9: secreted. using this antibody. Is that expected? Could you tell me where is MUC1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
From literature, mammary carcinoma does express MUC1. Generally MUC1 expresses in apical cell membrane, isoform 5: secreted., isoform y: secreted., isoform 9: secreted., mucin-1 subunit beta: cell membrane. Regarding which tissues have MUC1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 15969018
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lung, Pubmed ID: 8604237
Mammary carcinoma, Pubmed ID: 1697589, 11341784
Pancreatic carcinoma, Pubmed ID: 2394722
Boster Scientific Support
Answered: 2020-04-21
Question
We have seen staining in human carcinoma. Do you have any suggestions? Is anti-MUC1 antibody supposed to stain carcinoma positively?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
From literature carcinoma does express MUC1. From Uniprot.org, MUC1 is expressed in body of stomach, pancreatic carcinoma, mammary carcinoma, carcinoma, cervix carcinoma, thyroid, lung, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have MUC1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 15969018
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lung, Pubmed ID: 8604237
Mammary carcinoma, Pubmed ID: 1697589, 11341784
Pancreatic carcinoma, Pubmed ID: 2394722
Boster Scientific Support
Answered: 2020-02-10
Question
We are currently using anti-MUC1 antibody PB9145 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
O. Huang
Verified customer
Asked: 2019-11-26
Answer
The anti-MUC1 antibody (PB9145) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-26
Question
I am interested in using your anti-MUC1 antibody for nicotinamide salvaging studies. Has this antibody been tested with western blotting on ovary cancer tissue? We would like to see some validation images before ordering.
R. Brown
Verified customer
Asked: 2015-07-28
Answer
I appreciate your inquiry. This PB9145 anti-MUC1 antibody is tested on ovary cancer tissue. It is guaranteed to work for IHC in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-07-28
