Mouse TFPI PicoKine® Quick ELISA Kit

TFPI ELISA kit, 120-minute protocol

Mouse TFPI PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Mouse Tfpi in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Info Summary

SKU: FEK1355
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Assay Time: 120 Minutes

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Mouse TFPI PicoKine® Quick ELISA Kit

See all TFPI products

SKU/Catalog Number

FEK1355

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse TFPI PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Mouse Tfpi in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse TFPI PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1355)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: L29-N306

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of FEK1355 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1355 is reactive to Tfpi in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

FEK1355 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of TFPI

TFPI (Tissue factor pathway inhibitor),also known as EPI, LACI or TFPI1, is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). The gene for TFPI is located on chromosome 2q31-q32.1, and has nine exons which span 70 kb. TFPI consists of a highly negatively-charged amino-terminus, three tandemly-linked Kunitz domains, and a highly positively-charged carboxy-terminus. TFPI contributes significantly to the inhibition of Xa in vivo, despite being present at concentrations of only 2.5 nM.

Kit Components

Catalog number Description Quantity
FEK1355-CAP 96-well plate precoated with anti-Mouse TFPI antibody 1
EK1355-ST lyophilized recombinant Mouse TFPI standard 10 ng/tube
FEK1355-DA Biotinylated anti-Mouse TFPI detection antibody (50x) 100 ul
- Avidin-Biotin-Peroxidase Complex (30x) 400 µl
AR1106-1 Sample diluent 30 ml
AR1106-2 Antibody diluent 12 ml
- Avidin-Biotin-Peroxidase Diluent 12 ml
AR1106-5 Wash Buffer (25x) 20 ml
AR1104 Color Developing Reagent (TMB) 10 ml
AR1105 Stop Solution 10 ml
PLA-SEA Plate Sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512500025000500010,000
O.D.0.0190.0680.1040.2190.3800.7691.2991.957

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse TFPI PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3101506435933016304238
Standard deviation20.1599.39217.9522.44123.88216.13
CV (%)6.5%6.6%5%6.8%7.6%5.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TFPI in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 13103243153123155.351.6%
Sample 21506141015291285143296.146.7%
Sample 343594343424639384221169.294%
*number of samples for each test n=16.

Gene/Protein Information For Tfpi (Source: Uniprot.Org, NCBI)

Uniprot ID

O54819

Gene ID

7035

Gene Name

Tfpi

Full Name

Tissue factor pathway inhibitor

Weight

34987 MW

Alternative Names

Tissue factor pathway inhibitor;TFPI;Extrinsic pathway inhibitor;EPI;Lipoprotein-associated coagulation inhibitor;LACI;Tfpi; TFPI EPI, LACI, TFI1, TFPI tissue factor pathway inhibitor tissue factor pathway inhibitor|anti-convertin|extrinsic pathway inhibitor|tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Tfpi, check out the Tfpi Infographic

Tfpi infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Tfpi: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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