Anti-MMP3 Antibody Picoband®

Product Name

Anti-MMP3 Antibody Picoband®

View all MMP3 Antibodies

SKU/Catalog Number

PB9267
PB0265 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

MMP3 (stromelysin-1) is a secreted matrix metalloproteinase that participates in extracellular matrix (ECM) remodeling and can be activated after release into the ECM (e.g., via plasmin cascade), supporting processes such as tissue repair, inflammation, and invasion biology (putative in disease-specific contexts). Assay context: Human-reactive antibody validated for Western blot; useful when tracking protease-associated ECM turnover signatures alongside basement-membrane markers such as LAMA1/Laminin and growth-factor carrier proteins such as IGFBP3 (IGFBP3 in the IGF transport complex is subject to proteolysis by matrix proteases in related pathways).

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MMP3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9267)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminal of human MMP3, different from the related mouse sequence by seven amino acids, and from the related rat sequence by ten amino acids.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9267 is reactive to MMP3 in Human

Observed Molecular Weight

22-24 kDa

Calculated molecular weight

54.0 kDa

Background of MMP3

Stromelysin-1, also known as matrix metalloproteinase-3 (MMP-3), is an enzyme that in humans is encoded by the MMP3 gene. It is mapped to 11q22.2. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix and during tissue remodeling in normal physiological processes, such as embryonic development and reproduction, as well as in disease processes, such as arthritis, and tumour metastasis. The MMP-3 enzyme degrades collagen types II, III, IV, IX, and X, proteoglycans, fibronectin, laminin, and elastin. In addition, MMP-3 can also activate other MMPs such as MMP-1, MMP-7, and MMP-9, rendering MMP-3 crucial in connective tissue remodeling. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9267 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: Human Placenta Tissue, U20S Whole Cell, HELA Whole Cell, PANC Whole Cell, COLO320 Whole Cell

Validation Images & Assay Conditions

See full target information MMP3

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