Anti-MUC13 Antibody Picoband™

MUC13 antibody

Boster Bio Anti-MUC13 Antibody Picoband™ catalog # A02674-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A02674-2
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-MUC13 Antibody Picoband™

View all MUC13 Antibodies

SKU/Catalog Number

A02674-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MUC13 Antibody Picoband™ catalog # A02674-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MUC13 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02674-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MUC13 recombinant protein (Position: E226-S312).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02674-2 is reactive to MUC13 in Human, Mouse, Rat

Applications

A02674-2 is guaranteed for ELISA, IHC, WB Boster Guarantee

Background of MUC13

Epithelial mucins, such as MUC13, are a family of secreted and cell surface glycoproteins expressed by ductal and glandular epithelial tissues. It is mapped to 3q21.2. Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by epithelial tissues in most animals. Mucins' key characteristic is their ability to form gels; therefore they are a key component in most gel-like secretions, serving functions from lubrication to cell signalling to forming chemical barriers. They often take an inhibitory role. Some mucins are associated with controlling mineralization, including nacre formation in mollusks,calcification in echinoderms and bone formation in vertebrates. They bind to pathogens as part of the immune system. Overexpression of the mucin proteins, especially MUC1, is associated with many types of cancer. Although some mucins are membrane-bound due to the presence of a hydrophobic membrane-spanning domain that favors retention in the plasma membrane, most mucins are secreted as principal components of mucus by mucous membranes or are secreted to become a component of saliva.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For MUC13 (Source: Uniprot.org, NCBI)

Uniprot ID

Q9H3R2

Gene ID

56667

Gene Name

MUC13

Full Name

Mucin-13

Weight

54.604kDa

Alternative Names

Down-regulated in colon cancer 1FLJ20063; DRCC1; MUC-13; mucin 13, cell surface associated; mucin 13, epithelial transmembrane; mucin-13; RECC MUC13 DRCC1, MUC-13 mucin 13, cell surface associated mucin-13|down-regulated in colon cancer 1|mucin 13, epithelial transmembrane

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MUC13, check out the MUC13 Infographic

MUC13 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MUC13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

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6 Customer Q&As for Anti-MUC13 Antibody Picoband™

Question

We are currently using anti-MUC13 antibody A02674-2 for rat tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-27

Answer

The anti-MUC13 antibody (A02674-2) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-27

Question

I am looking for to test anti-MUC13 antibody A02674-2 on rat colon for research purposes, then I may be interested in using anti-MUC13 antibody A02674-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

L. Collins

Verified customer

Asked: 2019-11-18

Answer

The products we sell, including anti-MUC13 antibody A02674-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-11-18

Question

Can you help my question with product A02674-2, anti-MUC13 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-11-06

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02674-2 anti-MUC13 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-11-06

Question

Will A02674-2 anti-MUC13 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-24

Answer

As indicated on the product datasheet, A02674-2 anti-MUC13 antibody as been validated on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-24

Question

Is this A02674-2 anti-MUC13 antibody reactive to the isotypes of MUC13?

Verified Customer

Verified customer

Asked: 2019-04-04

Answer

The immunogen of A02674-2 anti-MUC13 antibody is E.coli-derived human MUC13 recombinant protein (Position: E226-S312). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-04-04

Question

Will anti-MUC13 antibody A02674-2 work for IHC-P with colon?

R. Anderson

Verified customer

Asked: 2016-07-22

Answer

According to the expression profile of colon, MUC13 is highly expressed in colon. So, it is likely that anti-MUC13 antibody A02674-2 will work for IHC-P with colon.

Boster Scientific Support

Answered: 2016-07-22

Size

Conjugation

Total: $315

SKU:A02674-2

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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A02674-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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