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Product Info Summary
| SKU: | PB9147 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MUC4 Antibody Picoband®
SKU/Catalog Number
PB9147
PB0157 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MUC4 Antibody Picoband® catalog # PB9147. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MUC4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9147)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MUC4 recombinant protein (Position: M1249-D1444). Human MUC4 shares 69% and 36% amino acid (aa) sequences identity with mouse and rat MUC4, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9147 is reactive to MUC4 in Human, Rat
Observed Molecular Weight
234 kDa
Calculated molecular weight
231.5 kDa
Background of MUC4
Mucin 4, also known as MUC4, is a mucin protein that in humans is encoded by the MUC4 gene. Like other mucins, Mucin 4 is a high-molecular weight glycoprotein. It is mapped to 3q29. In normal functioning, Mucin 4 is known to play anti-adhesive roles in the body, such as in lubricating the reproductive lining. It is found in the respiratory tract, particularly in the trachea and lung and the digestive tract, in the esophagus and colon, as well as in the visual and auditory systems. Mucin 4 serves to protect and lubricate the epithelium, which facilitates transport and traps foreign particles. It is found to be overexpressed during the luteal phase of menstruation. Mucin 4 may also have a role in fetal morphogenic development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9147 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat, Human
Positive Control
WB: rat PC-12 whole cell
Validation Images & Assay Conditions
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Western blot analysis of MUC4 using anti-MUC4 antibody (PB9147).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC4 antigen affinity purified polyclonal antibody (Catalog # PB9147) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUC4 at approximately 234 kDa. The expected band size for MUC4 is at 232 kDa.
Specific Publications For Anti-MUC4 Antibody Picoband® (PB9147)
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4 Customer Q&As for Anti-MUC4 Antibody Picoband®
Question
We are currently using anti-MUC4 antibody PB9147 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
The anti-MUC4 antibody (PB9147) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-17
Question
I am interested in using your anti-MUC4 antibody for dectin-2 family studies. Has this antibody been tested with western blotting on pc12 whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
Thanks for your inquiry. This PB9147 anti-MUC4 antibody is validated on pc12 whole cell lysate. It is guaranteed to work for WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-15
Question
We have observed staining in human colon mucosa. What should we do? Is anti-MUC4 antibody supposed to stain colon mucosa positively?
Verified Customer
Verified customer
Asked: 2019-01-02
Answer
From literature colon mucosa does express MUC4. From Uniprot.org, MUC4 is expressed in nasal cavity epithelium, lung testis, pancreatic tumor, colon mucosa, milk, among other tissues. Regarding which tissues have MUC4 expression, here are a few articles citing expression in various tissues:
Colon mucosa, Pubmed ID: 10024507
Lung, and Testis, Pubmed ID: 10880978
Milk, Pubmed ID: 18780401
Pancreatic tumor, Pubmed ID: 10920259
Boster Scientific Support
Answered: 2019-01-02
Question
My boss were satisfied with the WB result of your anti-MUC4 antibody. However we have observed positive staining in nasal cavity epithelium membrane using this antibody. Is that expected? Could you tell me where is MUC4 supposed to be expressed?
P. Williams
Verified customer
Asked: 2018-08-30
Answer
Based on literature, nasal cavity epithelium does express MUC4. Generally MUC4 expresses in membrane. Regarding which tissues have MUC4 expression, here are a few articles citing expression in various tissues:
Colon mucosa, Pubmed ID: 10024507
Lung, and Testis, Pubmed ID: 10880978
Milk, Pubmed ID: 18780401
Pancreatic tumor, Pubmed ID: 10920259
Boster Scientific Support
Answered: 2018-08-30
