|Validated Species:||Human, Rat|
Data & Images
|Product Name||Anti-MUC4 Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Mucin-4(MUC4) detection. Tested with WB in Human;Rat.|
|Cite This Product||Anti-MUC4 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9147)|
|Replacement Item||This antibody may replace the following items: sc-162941|sc-134695|sc-162940 from Santa Cruz Biotechnology.|
|Validated Species||Human, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human MUC4 recombinant protein (Position: M1249-D1444). Human MUC4 shares 69% and 36% amino acid (aa) sequences identity with mouse and rat MUC4, respectively.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||231518 MW|
|Protein Function||May play a role in tumor progression. Ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. Has anti-adhesive properties. Seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. Plays an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 'Tyr-1248'. In polarized epithelilal cells segragates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. .|
|Tissue Specificity||Expressed in the thymus, thyroid, lung, trachea, esophagus, stomach, small intestine, colon, testis, prostate, ovary, uterus, placenta, and mammary and salivary glands. Expressed in carcinomas arising from some of these epithelia, such as lung cancers, squamous cell carcinomas of the upper aerodigestive tract, mammary carcinomas, biliary tract, colon, and cervix cancers. Minimally or not expressed in the normal pancreas or chronic pancreatitis, but is highly expressed in pancreatic tumors and pancreatic tumor cell lines. .|
|Sequence Similarities||Contains 1 AMOP domain.|
|Subcellular Localization||Membrane ; Single-pass membrane protein . Secreted . Isoforms lacking the Cys-rich region, EGF-like domains and transmembrane region are secreted. Secretion occurs by splicing or proteolytic processing.|
|Alternative Names||Mucin-4;MUC-4;Ascites sialoglycoprotein;ASGP;Pancreatic adenocarcinoma mucin;Testis mucin;Tracheobronchial mucin;Mucin-4 alpha chain;Ascites sialoglycoprotein 1;ASGP-1;Mucin-4 beta chain;Ascites sialoglycoprotein 2;ASGP-2;MUC4;|
|Research Areas|||microbiology|protein|human protein|defensin| signal transduction|cytoskeleton / ecm|extracellular matrix|ecm proteins|muc| cancer|invasion/microenvironment|tumor immunology|tumor-associated antigens|tumor biomarkers|tumor antigens||
Background for Mucin-4
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-MUC4 Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Rat, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-MUC4 Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti MUC4 (PB9147) at 0.5ug/ml
WB: PC12 Whole Cell Lysate at 40ug
Predicted bind size: 234KD
Observed bind size: 234KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,