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Product Info Summary
| SKU: | MA1062 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC |
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Product info
Product Name
Anti-Myoglobin Mb Antibody (Monoclonal, MG-1)
SKU/Catalog Number
MA1062
BM0095 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Myoglobin Mb Antibody (Monoclonal, MG-1) catalog # MA1062. Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Myoglobin Mb Antibody (Monoclonal, MG-1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1062)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
MG-1
Isotype
Mouse IgG1
Immunogen
Purified human skeletal muscle myoglobin.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1062 is reactive to Mb in Human
Calculated molecular weight
17.2 kDa
Background of Mb
Human myoglobin has 152 residues. Two myoglobin variants were found. The myoglobin locus mapped to 22q11-22q13. The myoglobin gene is about 10.5 kb long and contains two introns as in the case with hemoglobin genes. Myoglobin may serve a variety of functions in muscular oxygen supply, such as O (2) storage, facilitated O (2) diffusion, and myoglobin-mediated oxidative phosphorylation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1062 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human
Positive Control
IHC: human tongue tissue
Validation Images & Assay Conditions
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IHC analysis of Myoglobin using anti-Myoglobin antibody (MA1062).
Myoglobin was detected in a paraffin-embedded section of human tongue tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Myoglobin Antibody (MA1062) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Specific Publications For Anti-Myoglobin Mb Antibody (Monoclonal, MG-1) (MA1062)
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Customer Reviews
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3 Customer Q&As for Anti-Myoglobin Mb Antibody (Monoclonal, MG-1)
Question
We are currently using anti-Myoglobin antibody (Monoclonal, MG-1) MA1062 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
The anti-Myoglobin antibody (Monoclonal, MG-1) (MA1062) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-25
Question
I am interested in using your anti-Myoglobin antibody (Monoclonal, MG-1) for response to hydrogen peroxide studies. Has this antibody been tested with western blotting on rat skeletal muscle tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
I appreciate your inquiry. This MA1062 anti-Myoglobin antibody (Monoclonal, MG-1) is validated on rat skeletal muscle tissue, tissue lysate. It is guaranteed to work for IHC in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-23
Question
We have been able to see staining in human heart right ventricle. Any tips? Is anti-Myoglobin antibody (Monoclonal, MG-1) supposed to stain heart right ventricle positively?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
According to literature heart right ventricle does express MB. According to Uniprot.org, MB is expressed in heart right ventricle, skeletal muscle, heart, among other tissues. Regarding which tissues have MB expression, here are a few articles citing expression in various tissues:
Heart, Pubmed ID: 7895732
Skeletal muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-05-30
