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Product Info Summary
| SKU: | PB10054 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB, ELISA (Cap) |
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Product info
Product Name
Anti-CXCL16 Antibody Picoband®
SKU/Catalog Number
PB10054
PB1110 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CXCL16 Antibody Picoband® catalog # PB10054. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CXCL16 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10054)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CXCL16 recombinant protein (Position: N30-A196). Human CXCL16 shares 47% and 45.7% amino acid (aa) sequence identity with mouse and rat CXCL16, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB10054 is reactive to CXCL16 in Human
Observed Molecular Weight
35 kDa
Calculated molecular weight
27.6 kDa
Background of CXCL16
CXCL16, Chemokine (C-X-C motif) ligand 16, is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines (with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. By somatic cell hybrid analysis, the CXCL16 gene is mapped to 17p13, a locus separate from all other known chemokines. Chemotaxis assays found that CXCL16 induced a strong chemotactic response in activated CD8 T cells. In addition, CXCL16 induced calcium mobilization.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10054 is guaranteed for WB, ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
ELISA(Cap) , 1-5μg/ml, Human, -
Positive Control
WB: CEM whole cell, A549 whole cell
Validation Images & Assay Conditions
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Western blot analysis of CXCL16 using anti-CXCL16 antibody (PB10054).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: CEM whole cell lysates,
Lane 2: A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL16 antigen affinity purified polyclonal antibody (Catalog # PB10054) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL16 at approximately 35 kDa. The expected band size for CXCL16 is at 28 kDa.
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Sandwich ELISA - Recombinant human cxcl16 protein standard curve.
Use in combination with reagents from Human cxcl16 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0741).
Specific Publications For Anti-CXCL16 Antibody Picoband® (PB10054)
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3 Customer Q&As for Anti-CXCL16 Antibody Picoband®
Question
Will anti-CXCL16 antibody PB10054 work for ELISA with spleen testis?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
According to the expression profile of spleen testis, CXCL16 is highly expressed in spleen testis. So, it is likely that anti-CXCL16 antibody PB10054 will work for ELISA with spleen testis.
Boster Scientific Support
Answered: 2019-06-24
Question
We are interested in to test anti-CXCL16 antibody PB10054 on human spleen testis for research purposes, then I may be interested in using anti-CXCL16 antibody PB10054 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
The products we sell, including anti-CXCL16 antibody PB10054, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-11
Question
We are currently using anti-CXCL16 antibody PB10054 for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-25
Answer
The anti-CXCL16 antibody (PB10054) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-25
