Product Info Summary
SKU: | PB10054 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CXCL16 Antibody Picoband™
SKU/Catalog Number
PB10054
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CXCL16 Antibody Picoband™ catalog # PB10054. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CXCL16 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10054)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CXCL16 recombinant protein (Position: N30-A196). Human CXCL16 shares 47% and 45.7% amino acid (aa) sequence identity with mouse and rat CXCL16, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB10054 is reactive to CXCL16 in Human
Applications
PB10054 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
35 kDa
Calculated molecular weight
27.579kDa
Background of CXCL16
CXCL16, Chemokine (C-X-C motif) ligand 16, is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines (with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. By somatic cell hybrid analysis, the CXCL16 gene is mapped to 17p13, a locus separate from all other known chemokines. Chemotaxis assays found that CXCL16 induced a strong chemotactic response in activated CD8 T cells. In addition, CXCL16 induced calcium mobilization.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
ELISA(Cap) , 1-5μg/ml, Human, -
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CXCL16 using anti-CXCL16 antibody (PB10054).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: CEM whole cell lysates,
Lane 2: A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL16 antigen affinity purified polyclonal antibody (Catalog # PB10054) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL16 at approximately 35 kDa. The expected band size for CXCL16 is at 28 kDa.
Click image to see more details
Figure 2. Sandwich ELISA - Recombinant human cxcl16 protein standard curve.
Use in combination with reagents from Human cxcl16 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0741).
Protein Target Info & Infographic
Gene/Protein Information For CXCL16 (Source: Uniprot.org, NCBI)
Gene Name
CXCL16
Full Name
C-X-C motif chemokine 16
Weight
27.579kDa
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
chemokine (C-X-C motif) ligand 16; CXC chemokine ligand 16; CXCL16; CXCLG16; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SCYB16; Small-inducible cytokine B16; SRPSOXC-X-C motif chemokine 16; SR-PSOXTransmembrane chemokine CXCL16 CXCL16 CXCLG16, SR-PSOX, SRPSOX C-X-C motif chemokine ligand 16 C-X-C motif chemokine 16|CXC chemokine ligand 16|chemokine (C-X-C motif) ligand 16|scavenger receptor for phosphatidylserine and oxidized low density lipoprotein|small-inducible cytokine B16|transmembrane chemokine CXCL16
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CXCL16, check out the CXCL16 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CXCL16: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CXCL16 Antibody Picoband™ (PB10054)
Hello CJ!
No publications found for PB10054
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CXCL16 Antibody Picoband™?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-CXCL16 Antibody Picoband™
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-CXCL16 Antibody Picoband™
Question
Will anti-CXCL16 antibody PB10054 work for ELISA with spleen testis?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
According to the expression profile of spleen testis, CXCL16 is highly expressed in spleen testis. So, it is likely that anti-CXCL16 antibody PB10054 will work for ELISA with spleen testis.
Boster Scientific Support
Answered: 2019-06-24
Question
We are interested in to test anti-CXCL16 antibody PB10054 on human spleen testis for research purposes, then I may be interested in using anti-CXCL16 antibody PB10054 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
The products we sell, including anti-CXCL16 antibody PB10054, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-11
Question
We are currently using anti-CXCL16 antibody PB10054 for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-25
Answer
The anti-CXCL16 antibody (PB10054) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-25