Human PTX3/Pentraxin 3 ELISA Kit PicoKine™

Human PTX3/Pentraxin 3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PTX3 in cell culture supernatants, serum, plasma (EDTA) and saliva. Sensitivity: 10pg/ml. Cited in 13 publication(s).

Product Info Summary

SKU: EK0861
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (EDTA) and saliva.

Product Name

Human PTX3/Pentraxin 3 ELISA Kit PicoKine™

See all Pentraxin 3/TSG-14 products

SKU/Catalog Number

EK0861

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human PTX3/Pentraxin 3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PTX3 in cell culture supernatants, serum, plasma (EDTA) and saliva. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human PTX3/Pentraxin 3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0861)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E18-S381

Sensitivity

<10 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0861 is reactive to PTX3 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (EDTA) and saliva.

Application Guarantee

EK0861 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0861-CAP Anti-Human PTX3 Pre-coated 96-well strip microplate 1
EK0861-ST Human PTX3 Standard 2 vials, 20 ng/tube
EK0861-DA Human PTX3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03126251250250050001000020000
O.D.0.0520.2780.4200.6491.1111.6051.9412.215

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human PTX3/Pentraxin 3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)6621701919471818169601
Standard deviation33.7685.05579.2238.7792.61662.46
CV (%)6.9%5%6.9%5.4%6.9%6.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Pentraxin 3/TSG-14 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 166262069360464434.995.4%
Sample 21701155615361612160163.973.9%
Sample 391948999894087638974153.861.7%
*number of samples for each test n=16.

Gene/Protein Information For PTX3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P26022

Gene ID

5806

Gene Name

PTX3

Full Name

Pentraxin-related protein PTX3

Weight

41.976kDa

Alternative Names

alpha-induced protein 5; pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor; Pentaxin-related protein PTX3; Pentraxin 3; pentraxin 3, long; pentraxin-3; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin-related protein PTX3; PTX3; TNF alpha-induced protein 5; TNFAIP5; TSG14; TSG-14; TSG-14pentaxin-related gene, rapidly induced by IL-1 beta; Tumor necrosis factor alpha-induced protein 5; tumor necrosis factor, alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; tumor necrosis factor-inducible protein TSG-14 PTX3 TNFAIP5, TSG-14 pentraxin 3 pentraxin-related protein PTX3|TNF alpha-induced protein 5|long pentraxin 3|tumor necrosis factor alpha-induced protein 5|tumor necrosis factor-inducible gene 14 protein|tumor necrosis factor-inducible protein TSG-14

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PTX3, check out the PTX3 Infographic

PTX3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PTX3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0861 has been cited in 13 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Moulana Z,Bagherzadeh M,Mirzakhani M,Rostami A,Mohammadnia-Afrouzi M,Shahbazi M.Increased Levels of serum Pentraxin 3 in Critical Coronavirus Disease-2019 Patients.Environ Sci Pollut Res Int.2021 Jul 1:1–5.doi:10.1007/s11356-021-15183-9.Epub ahead of print.PMID:34212320;PMCID:PMC8248285.
Species: Human
EK0861 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Hussien HA,Habieb MS,Hamdan AM.Evaluation of Serum Total Immunoglobulin E, Interleukin-17 and Pentraxin-3 as Biomarkers for Chronic Rhinosinusitis With Nasal Polyposis.Am J Rhinol Allergy.2020 Dec 23:1945892420983787.doi:10.1177/1945892420983787.Epub ahea
Species: Human
EK0861 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Dongel, I., Gokmen, A. A., Camas, H. E., Gonen, I., & Kaya, S. (2020). Diagnostic significance of biochemical markers and pentraxin-3 in the differential diagnosis of malign, benign pleural effusion and empyema. JPMA, 2020.

Investigation of the Levels of Serum Amyloid A, YKL-40, and Pentraxin-3 in Patients with Familial Mediterranean Fever

Pentraxin-3 and inflammatory biomarkers related to posterolateral thoracotomy in Thoracic Surgery

Pentraxin-3: A strong novel biochemical marker for appendicitis in children

Changes of serum pentraxin-3 and hypersensitive CRP levels during pregnancy and their relationship with gestational diabetes mellitus

New Biomarkers to Diagnose Ventilator Associated Pneumonia: Pentraxin 3 and Surfactant Protein D

The Relationship Between Serum Pentraxine 3 Levels and Hematological Markers in Patients With Rheumatoid Arthritis

Sequential Measurements of Pentraxin 3 Serum Levels in Patients with Ventilator-Associated Pneumonia: A Nested Case-Control Study

Comparative evaluation of pentraxin 3 levels in GCF during canine retraction with active tieback and NiTi coil spring: An in vivo study

Serum Copeptin, Pentraxin 3, Anti-Mullerian Hormone Levels With Echocardiography and Carotid Artery Intima-Media Thickness in Adolescents With Polycystic Ovary Syndrome

Kim Hs, Won S, Lee Ek, Chun Yh, Yoon Js, Kim Hh, Kim Jt. Pediatr Pulmonol. 2016 Jan;51(1):42-8. Doi: 10.1002/Ppul.23199. Epub 2015 Apr 1. Pentraxin 3 As A Clinical Marker In Children With Lower Respiratory Tract Infection.

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Find answers in Q&As, reviews.

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12 Customer Q&As for Human PTX3/Pentraxin 3 ELISA Kit PicoKine™

Question

Can MNB4 antibody be suitable in the measurement of the PTX3?

Verified customer

Asked: 2020-12-09

Answer

Unfortunatley, our lab hasn't used antibody MNB4 in the measurement of the PTX3 and we are uncertian if could be used.

Boster Scientific Support

Answered: 2020-12-09

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

M. Green

Verified customer

Asked: 2020-06-24

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-06-24

Question

Can the Human PTX3/Pentraxin 3 PicoKine™ ELISA Kit be used on heparinized plasma?

Verified Customer

Verified customer

Asked: 2019-09-24

Answer

The Human PTX3/Pentraxin 3 PicoKine™ ELISA Kit has been validated with plasma samples using EDTA as a coagulant. Serum and heparin plasma are not suitable for use in this assay.

Boster Scientific Support

Answered: 2019-09-24

Question

Q: is it okay to use edta plasma as samples in Human Pentraxin 3 Picokine® ELISA Kit (Catalog # EK0861)?

R. Zhou

Verified customer

Asked: 2019-09-12

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Pentraxin 3 causing degradation of its protein structure. Pentraxin 3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Pentraxin 3 ELISA, treating samples with various anticoagulants and decided that EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-09-12

Question

Q: Can Pentraxin 3 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

T. Singh

Verified customer

Asked: 2019-02-19

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-02-19

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2018-12-28

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-12-28

Question

Q: how to proceed with the analysis of ELISA data? I have obtained Pentraxin 3 level in plasma.

Verified Customer

Verified customer

Asked: 2018-06-24

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-06-24

Question

Q: What is the optimal O.D. value for Pentraxin 3 ELISA kit? I performed your Pentraxin 3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Pentraxin 3 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-06-10

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Pentraxin 3 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Pentraxin 3 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-06-10

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2018-06-10

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-06-10

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Pentraxin 3 in Human plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2017-12-30

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Pentraxin 3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2017-12-30

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Pentraxin 3 using the EK0861 Human Pentraxin 3 Picokine® ELISA Kit?

R. Zhou

Verified customer

Asked: 2017-11-27

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2017-11-27

Question

How is the standard derived? Keyword: expression vector

Verified Customer

Verified customer

Asked: 2013-01-22

Answer

The standard is derived from mouse myeloma cell line, and there is no viral component.

Boster Scientific Support

Answered: 2013-01-22

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