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- Table of Contents
NOX4 antibody (A00403) performs well in IF, ICC, WB and shows reactivity with Human, Monkey.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 10 Publications
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IF analysis of NOX4 using anti-NOX4 antibody (A00403).
NOX4 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NOX4 Antibody (A00403) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
NOX4 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NOX4 Antibody (A00403) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-NADPH oxidase 4/NOX4 Antibody Picoband®
NOX4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey | Rabbit | IF, ICC, WB |
Tested Applications
NOX4 antibody was positively detected by WB in:
human 293T whole cell, human U87 whole cell, human SH-SY5Y whole cell, human U251 whole cell, human U2OS whole cell, human Hela whole cell, human T47D whole cell, monkey COS-7 whole cell, human BEAS-2B whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
NOX4 antibody was positively detected by ICC/IF in:
U2OS cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence
5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
Human
Tested
Monkey
Tested
Bovine
Predicted
Dog
Predicted
Mouse
Predicted
Rat
Predicted
Rabbit
Predicted
Sheep
Predicted
Product Information
Product Name
Anti-NADPH oxidase 4/NOX4 Antibody Picoband®
View all NOX4 Antibodies
View all NOX4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human NADPH oxidase 4, which shares 97.2% amino acid (aa) sequence identity with both mouse and rat NADPH oxidase 4.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65 kDa
Calculated Molecular Weight
66.9 kDa
Background of NOX4
NADPH oxidase 4 is an enzyme that in humans is encoded by the NOX4 gene, and is a member of the NOX family of NADPH oxidases. This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.
Cite This Product
Anti-NADPH oxidase 4/NOX4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00403)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Cow
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Mouse
97%
High Homology
Rat
97%
High Homology
Rabbit
97%
High Homology
Sheep
97%
High Homology
Zebrafish
69%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for NOX4 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for NOX4 antibody
Publications
Application:
Species:
Exploring Liraglutide’s mechanism in reducing renal fibrosis: the Fsp1-CoQ10-NAD(P)H pathway
Published: 2025-01-12
Journal: Scientific Reports
Application: WB
Impact Factor: 3.90
Species: Mouse
PMID: 39799153
Synergistic Activation of LEPR and ADRB2 Induced by Leptin Enhances Reactive Oxygen Specie Generation in Triple-Negative Breast Cancer Cells
Published: 2024-08-21
Journal: BREAST CANCER RESEARCH AND TREATMENT
Application: WB
Impact Factor: 3.00
Species: Mouse
PMID: 39164083
Liraglutide attenuates hepatic iron levels and ferroptosis in db/db mice
Published: 2022-03-21
Journal: Bioengineered
Application: WB
Impact Factor: 6.83
Species: Human,Mouse
PMID: 35311455
Glucagon-like peptide-1 receptor activation by liraglutide promotes breast cancer through NOX4/ROS/VEGF pathway
Published: 2022-02-03
Journal: LIFE SCIENCES
Application: WB,IHC
Impact Factor: 6.78
Species: Human,Mouse
PMID: 35124000
Naringin Reverses High-Cholesterol Diet-Induced Vascular Dysfunction and Oxidative Stress in Rats via Regulating LOX-1 and NADPH Oxidase Subunit Expression
Published: 2019-10-24
Journal: Biomed Research International
Application: WB
Impact Factor: 2.20
Species: Rat
PMID: 31781614
Protective effect of peptide DR8 on bleomycin-induced pulmonary fibrosis by regulating the TGF-β/MAPK signaling pathway and oxidative stress
Published: 2019-08-06
Journal: TOXICOLOGY AND APPLIED PHARMACOLOGY
Application: WB
Impact Factor: 3.59
Species: Mouse,Human
PMID: 31398421
NADPH Oxidase Hyperactivity Contributes to Cardiac Dysfunction and Apoptosis in Rats with Severe Experimental Pancreatitis through ROS-Mediated MAPK Signaling Pathway
Published: 2019-05-09
Journal: Oxidative Medicine and Cellular Longevity
Application: IHC
Impact Factor: 4.87
Species: Rat
PMID: 31210840
Suppression of Oxidative Stress and Apoptosis in Electrically Stimulated Neonatal Rat Cardiomyocytes by Resveratrol and Underlying Mechanisms
Published: 2017-12-01
Journal: JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
Application: WB
Impact Factor: 2.25
Species: Rat
PMID: 28857948
Polydatin protects against carbon tetrachloride-induced liver fibrosis in mice
Published: 2017-06-27
Journal: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Application: IHC
Impact Factor: 3.17
PMID: 28666738
Bletilla striata polysaccharide inhibits angiotensin II-induced ROS and inflammation via NOX4 and TLR2 pathways
Published: 2016-05-03
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: WB
Impact Factor: 3.14
Species: Human
PMID: 27151672
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Product Promise
We promise Anti-NADPH oxidase 4/NOX4 Antibody Picoband® performs excellently in relevant applications for IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00403
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026