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Product Info Summary
| SKU: | M05685-3 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Napsin-A NAPSA Rabbit Monoclonal Antibody, Clone#RM366
SKU/Catalog Number
M05685-3
Size
100uL
Form
Liquid
Description
Boster Bio Anti-Napsin-A NAPSA Rabbit Monoclonal Antibody, Clone#RM366 (Catalog # M05685-3). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Napsin-A NAPSA Rabbit Monoclonal Antibody, Clone#RM366 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05685-3)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM366
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the N-terminus of human Napsin-A
Cross-reactivity
This antibody reacts human Napsin-A
Reactive Species
M05685-3 is reactive to NAPSA in Human
Calculated molecular weight
45.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05685-3 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:1000-1:2000 dilution
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of human lung tissue lysate using anti-Napsin-A rabbit monoclonal antibody (Clone RM366) at a 1:2000 dilution.
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Western Blotting result
Western Blot of human lung tissue lysate using anti-Napsin-A rabbit monoclonal antibody (Clone RM366) at a 1:2000 dilution.
Specific Publications For Anti-Napsin-A NAPSA Rabbit Monoclonal Antibody, Clone#RM366 (M05685-3)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Napsin-A NAPSA Rabbit Monoclonal Antibody, Clone#RM366
Question
I was wanting to use your anti-Napsin-A Rabbit Monoclonal antibody, Clone#RM366 for WB for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
It shows on the product datasheet, M05685-3 anti-Napsin-A Rabbit Monoclonal antibody, Clone#RM366 has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-01
Question
Will M05685-3 anti-Napsin-A Rabbit Monoclonal antibody, Clone#RM366 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
As indicated on the product datasheet, M05685-3 anti-Napsin-A Rabbit Monoclonal antibody, Clone#RM366 as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-18
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Napsin-A Rabbit Monoclonal antibody, Clone#RM366 M05685-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-19
Question
Does anti-Napsin-A Rabbit Monoclonal antibody, Clone#RM366 M05685-3 work on horse WB with upper lobe of lung?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
Our lab technicians have not tested anti-Napsin-A Rabbit Monoclonal antibody, Clone#RM366 M05685-3 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Napsin-A Rabbit Monoclonal antibody, Clone#RM366 M05685-3 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse upper lobe of lung in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-15
Question
Is a blocking peptide available for product anti-Napsin-A Rabbit Monoclonal antibody, Clone#RM366 (M05685-3)?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
We do provide the blocking peptide for product anti-Napsin-A Rabbit Monoclonal antibody, Clone#RM366 (M05685-3). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-06-05
Question
We are currently using anti-Napsin-A Rabbit Monoclonal antibody, Clone#RM366 M05685-3 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
T. Huang
Verified customer
Asked: 2017-07-21
Answer
The anti-Napsin-A Rabbit Monoclonal antibody, Clone#RM366 (M05685-3) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-21
Question
I see that the anti-Napsin-A Rabbit Monoclonal antibody, Clone#RM366 M05685-3 works with WB, what is the protocol used to produce the result images on the product page?
L. Li
Verified customer
Asked: 2013-11-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2013-11-15
