Anti-NEK5 Antibody

Nek5 antibody

Boster Bio Anti-NEK5 Antibody (Catalog # A14002). Tested in WB, IHC, IF applications. This antibody reacts with Human.

Product Info Summary

SKU: A14002
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: IF, IHC, WB

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Product Name

Anti-NEK5 Antibody

View all Nek5 Antibodies

SKU/Catalog Number

A14002

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-NEK5 Antibody (Catalog # A14002). Tested in WB, IHC, IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NEK5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14002)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human NEK5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A14002 is reactive to Nek5 in Human

Applications

A14002 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

71.79kDa

Background of Nek5

Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.

Dunham A., Nature 428:522-528(2004).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Molina H., Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For Nek5 (Source: Uniprot.org, NCBI)

Gene Name

Nek5

Full Name

Serine/threonine-protein kinase Nek5

Weight

71.79kDa

Superfamily

protein kinase superfamily

Alternative Names

never in mitosis A-related kinase 5; NIMA (never in mitosis gene a)-related kinase 5; nimA-related protein kinase 5; serine/threonine-protein kinase Nek5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Nek5, check out the Nek5 Infographic

Nek5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Nek5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-NEK5 Antibody

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bronchial epithelial cell using anti-NEK5 antibody A14002. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-17

Question

I was wanting to use your anti-NEK5 antibody for WB for human bronchial epithelial cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human bronchial epithelial cell identification?

Verified Customer

Verified customer

Asked: 2019-05-23

Answer

As indicated on the product datasheet, A14002 anti-NEK5 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human bronchial epithelial cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-23

Question

Is there a BSA free version of anti-NEK5 antibody A14002 available?

Verified Customer

Verified customer

Asked: 2018-11-23

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-NEK5 antibody A14002 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-11-23

Question

Does A14002 anti-NEK5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

K. Anderson

Verified customer

Asked: 2018-10-11

Answer

As indicated on the product datasheet, A14002 anti-NEK5 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-10-11

Question

My question regarding product A14002, anti-NEK5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

W. Huang

Verified customer

Asked: 2018-08-27

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14002 anti-NEK5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-08-27

Question

I am interested in to test anti-NEK5 antibody A14002 on human bronchial epithelial cell for research purposes, then I may be interested in using anti-NEK5 antibody A14002 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

C. Edwards

Verified customer

Asked: 2015-01-27

Answer

The products we sell, including anti-NEK5 antibody A14002, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2015-01-27

Order DetailsPrice
A14002-100uL

100uL

$360

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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