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Product Info Summary
| SKU: | M04660 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-NEK9 Mouse Monoclonal Antibody [Clone ID: OTI1H1]
SKU/Catalog Number
M04660
Size
100 µl
Description
Boster Bio NEK9 mouse monoclonal antibody, clone OTI1H1 (formerly 1H1). Catalog# M04660. Tested in FC, IF, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-NEK9 Mouse Monoclonal Antibody [Clone ID: OTI1H1] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04660)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1H1
Isotype
IgG1
Immunogen
Full length human recombinant protein of human NEK9 (NP_149107) produced in HEK293 cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04660 is reactive to NEK9 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
107 kDa
Calculated molecular weight
107.168kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04660 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK9.
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Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-NEK9 monoclonal antibody (1:200).
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HEK293T cells transfected with either NEK9 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NEK9 antibody (M04660)
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Anti-NEK9 mouse monoclonal antibody (M04660) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK9.
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and NEK9-Knockout hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-NEK9 monoclonal antibody M04660. Then the blotted membrane was stripped and reprobed with anti-β-actin as a loading control (1:500).
Protein Target Info & Infographic
Gene/Protein Information For NEK9 (Source: Uniprot.org, NCBI)
Gene Name
NEK9
Full Name
Serine/threonine-protein kinase Nek9
Weight
107.168kDa
Superfamily
protein kinase superfamily
Alternative Names
DKFZp434D0935; KIAA1995; MGC138306; MGC16714; NEK8; Nek8NERCC1; Nercc1 kinase; NERCCEC 2.7.11.1; Never in mitosis A-related kinase 9; NIMA (never in mitosis gene a)- related kinase 9; NimA-related kinase 8; NIMA-related kinase Nek8; NimA-related protein kinase 9; serine/threonine-protein kinase Nek9 NEK9 APUG, LCCS10, NC, NERCC, NERCC1 NIMA related kinase 9 serine/threonine-protein kinase Nek9|NIMA (never in mitosis gene a)- related kinase 9|nercc1 kinase|nimA-related protein kinase 9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NEK9, check out the NEK9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NEK9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NEK9 Mouse Monoclonal Antibody [Clone ID: OTI1H1] (M04660)
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