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Product Info Summary
| SKU: | A14597-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Neurexophilin-3 NXPH3 Antibody
SKU/Catalog Number
A14597-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Neurexophilin-3 NXPH3 Antibody catalog # A14597-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Neurexophilin-3 NXPH3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14597-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human NXPH3. AA range:158-207
Reactive Species
A14597-1 is reactive to NXPH3 in Human, Mouse, Rat
Calculated molecular weight
28.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14597-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Neurexophilin-3 Polyclonal Antibody
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Western blot analysis of lysates from HT-29 cells, using NXPH3 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HepG2 cells using NXPH3 antibody.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Specific Publications For Anti-Neurexophilin-3 NXPH3 Antibody (A14597-1)
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Customer Q&As
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6 Customer Q&As for Anti-Neurexophilin-3 NXPH3 Antibody
Question
Can you help my question with product A14597-1, anti-Neurexophilin-3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Carter
Verified customer
Asked: 2019-04-18
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14597-1 anti-Neurexophilin-3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-04-18
Question
I see that the anti-Neurexophilin-3 antibody A14597-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-03-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-03-19
Question
you antibody to test anti-Neurexophilin-3 antibody A14597-1 on rat hypothalamus for research purposes, then I may be interested in using anti-Neurexophilin-3 antibody A14597-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-05-30
Answer
The products we sell, including anti-Neurexophilin-3 antibody A14597-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-05-30
Question
Is a blocking peptide available for product anti-Neurexophilin-3 antibody (A14597-1)?
J. Johnson
Verified customer
Asked: 2016-10-28
Answer
We do provide the blocking peptide for product anti-Neurexophilin-3 antibody (A14597-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2016-10-28
Question
We are currently using anti-Neurexophilin-3 antibody A14597-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
P. Jones
Verified customer
Asked: 2015-05-06
Answer
The anti-Neurexophilin-3 antibody (A14597-1) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-05-06
Question
Do you have a BSA free version of anti-Neurexophilin-3 antibody A14597-1 available?
K. Jackson
Verified customer
Asked: 2013-05-30
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Neurexophilin-3 antibody A14597-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-05-30
