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Product Info Summary
| SKU: | A08120-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-NIP7 Antibody Picoband®
SKU/Catalog Number
A08120-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NIP7 Antibody Picoband® catalog # A08120-2. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NIP7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08120-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human NIP7 recombinant protein (Position: E14-T180). Human NIP7 shares 97.6% and 97% amino acid (aa) sequence identity with mouse and rat NIP7, respectively.
Reactive Species
A08120-2 is reactive to NIP7 in Human, Mouse
Observed Molecular Weight
20 kDa
Calculated molecular weight
20.5 kDa
Background of NIP7
60S ribosome subunit biogenesis protein NIP7 homolog is a protein that in humans is encoded by the NIP7 gene. NIP7 is required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08120-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human 293T whole cell, human SIHA whole cell, mouse NIH/3T3 whole cell
FCM: 293T cell
Validation Images & Assay Conditions
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Western blot analysis of NIP7 using anti-NIP7 antibody (A08120-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human SIHA whole cell lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NIP7 antigen affinity purified polyclonal antibody (Catalog # A08120-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NIP7 at approximately 20 kDa. The expected band size for NIP7 is at 20 kDa.
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Flow Cytometry analysis of 293T cells using anti-NIP7 antibody (A08120-2).
Overlay histogram showing 293T cells stained with A08120-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NIP7 Antibody (A08120-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Specific Publications For Anti-NIP7 Antibody Picoband® (A08120-2)
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