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Product Info Summary
| SKU: | A10985 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Nop132 NOL8 Antibody
SKU/Catalog Number
A10985
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Nop132 NOL8 Antibody catalog # A10985. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nop132 NOL8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10985)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human NOL8. AA range:1118-1167
Reactive Species
A10985 is reactive to NOL8 in Human, Mouse
Calculated molecular weight
131.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10985 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot (WB) analysis of NIH-3T3 cells using Nop132 Polyclonal antibody.
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Western Blot (WB) analysis of 3T3 cells using Nop132 Polyclonal antibody.
Specific Publications For Anti-Nop132 NOL8 Antibody (A10985)
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5 Customer Q&As for Anti-Nop132 NOL8 Antibody
Question
Will anti-Nop132 antibody A10985 work for WB with embl:aah13788.2}?
L. Patel
Verified customer
Asked: 2020-04-29
Answer
According to the expression profile of embl:aah13788.2}, NOL8 is highly expressed in embl:aah13788.2}. So, it is likely that anti-Nop132 antibody A10985 will work for WB with embl:aah13788.2}.
Boster Scientific Support
Answered: 2020-04-29
Question
See attached the WB image, lot number and protocol we used for embl:aah13788.2} using anti-Nop132 antibody A10985. Please let me know if you require anything else.
E. Jha
Verified customer
Asked: 2018-02-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-05
Question
My question regarding product A10985, anti-Nop132 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-06-19
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10985 anti-Nop132 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-19
Question
We are currently using anti-Nop132 antibody A10985 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2017-05-12
Answer
The anti-Nop132 antibody (A10985) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-12
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embl:aah13788.2} using anti-Nop132 antibody A10985. Let me know if you need anything else.
J. Parker
Verified customer
Asked: 2017-05-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-09
