Product Info Summary
SKU: | A01206-2 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Monkey, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-NUMB Antibody Picoband™
SKU/Catalog Number
A01206-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NUMB Antibody Picoband™ catalog # A01206-2. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NUMB Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01206-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Clone Number
1B9
Isotype
IgG
Immunogen
E.coli-derived human NUMB recombinant protein (Position: E59-A567). Human NUMB shares 93.7% amino acid (aa) sequence identity with both mouse and rat NUMB.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01206-2 is reactive to NUMB in Human, Monkey, Mouse, Rat
Applications
A01206-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
72-75 kDa
Calculated molecular weight
70.804kDa
Background of NUMB
Protein numb homolog is a protein that in humans is encoded by the NUMB gene. It is mapped to 14q24.2 to q24.3. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The primary function of Numb in cell differentiation is as an inhibitor of Notch signaling which is essential for maintaining self-renewal potential in stem and progenitor cells. Numb also plays a crucial role in asymmetrical cell division during development, allowing for differential cell fate specification in the central and peripheral nervous systems. What’s more, the numb gene protein product controls binary cell fate decisions in the peripheral and central nervous systems of both invertebrates and mammals during neurogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Monkey, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NUMB using anti-NUMB antibody (A01206-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U-87 MG whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: rat brain tissue tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUMB antigen affinity purified polyclonal antibody (Catalog # A01206-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUMB at approximately 72-75 kDa. The expected band size for NUMB is at 42 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of RT4 cells using anti-NUMB antibody (A01206-2).
Overlay histogram showing RT4 cells stained with A01206-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUMB Antibody (A01206-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For NUMB (Source: Uniprot.org, NCBI)
Gene Name
NUMB
Full Name
Protein numb homolog
Weight
70.804kDa
Alternative Names
h-Numb; mnb; m-numb; numb (Drosophila) homolog; numb homolog (Drosophila); Numb; protein numb homolog; Protein S171; S171 NUMB C14orf41, S171, c14_5527 NUMB endocytic adaptor protein protein numb homolog|h-Numb|numb homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NUMB, check out the NUMB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NUMB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NUMB Antibody Picoband™ (A01206-2)
Hello CJ!
No publications found for A01206-2
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-NUMB Antibody Picoband™?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-NUMB Antibody Picoband™
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question