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- Table of Contents
OCLN antibody (A01246-4) performs well in ELISA, IP, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 14 Publications
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IHC analysis of Occludin/OCLN using anti-Occludin/OCLN antibody (A01246-4).
Occludin/OCLN was detected in a paraffin-embedded section of human stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Occludin/OCLN Antibody (A01246-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Occludin/OCLN was detected in a paraffin-embedded section of human stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Occludin/OCLN Antibody (A01246-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Occludin/OCLN Antibody Picoband®
OCLN antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IP, IHC, WB |
Tested Applications
OCLN antibody was positively detected by WB in:
human HepG2 whole cell, human 293T whole cell, human Jurkat whole cell, rat liver tissue, mouse liver tissue, mouse kidney tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
OCLN antibody was positively detected by IP in:
HepG2 whole cell
OCLN antibody was positively detected by IHC in:
human stomach tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunoprecipitation
0.5-2 μg/ml
Human
ELISA
0.1-0.5 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Occludin/OCLN Antibody Picoband®
View all OCLN Antibodies
View all OCLN Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human Occludin/OCLN recombinant protein (Position: S358-Q520). Human Occludin/OCLN shares 88.8% and 88.2% amino acid (aa) sequence identity with mouse and rat Occludin/OCLN, respectively.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
59 kDa
Calculated Molecular Weight
59.1 kDa
Background of OCLN
Occludin is an integral membrane protein that is located at tight junctions. It is a member of a family of proteins containing the highly conserved Marvel domain, which contains 4 transmembrane-helix regions. This gene is mapped to 5q13.2. Occludin regulates TGF-beta receptor type I localization for efficient TGF-beta-dependent dissolution of tight junctions during epithelial-mesenchymal transitions. Human Occludin is an essential hepatitis C virus (HCV) cell entry factor that is able to render murine cells infectable with HCV glycoproteins. It has been found that occludin is involved in cell migration and functions to recruit active PI3 kinase to the leading edge, resulting in RAC1 activation and formation of lamellipodia.
Cite This Product
Anti-Occludin/OCLN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01246-4)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Dog
91%
High Homology
Cow
91%
High Homology
Rabbit
90%
High Homology
Sheep
90%
High Homology
Mouse
88%
Moderate Homology
Rat
87%
Moderate Homology
Zebrafish
44%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for OCLN antibody
Immunoprecipitation (IP) protocol for OCLN antibody
Immunohistochemistry (IHC) protocol for OCLN antibody
Publications
Application:
Species:
Wendan decoction modulates Parasutterella to influence fatty acid metabolism in MAFLD via the FXR/PPARα/CYP4A12A axis
Published: 2026-07-20
Journal: Chinese Medicine
Application: IHC,WB
Impact Factor: 7.40
Species: Mouse
PMID: 42477798
Betulinic Acid Enema Alleviates Colitis by Targeting Vitamin D Receptor to Protect the Intestinal Mucosal Barrier and Modulate Mucosa-Associated Microbiota
Published: 2026-06-18
Journal: PHYTOTHERAPY RESEARCH
Application: IHC,IF,WB
Impact Factor: 8.10
Species: Mouse,Human
PMID: 42316556
Resveratrol Attenuates Neuroinflammation and Gut–Brain Axis Dysfunction Induced by Lead and Cadmium Co-Exposure by Modulating Gut Microbiota in Rats
Published: 2026-03-16
Journal: PHYTOTHERAPY RESEARCH
Application: WB
Impact Factor: 6.30
Species: Rat
PMID: 41839449
Electroacupuncture Prevents Against AD‐Like Phenotypes in APP/PS1 Mice: Investigation of the Mechanisms From Cerebral Microangiopathy
Published: 2025-12-09
Journal: CNS Neuroscience & Therapeutics
Application: WB
Impact Factor: 5.00
Species: Mouse
PMID: 41367129
Medulla Tetrapanacis Water Extract Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis by Reprogramming Macrophage Polarization and Maintaining Intestinal Barrier Integrity
Published: 2025-10-29
Journal: Food Frontiers
Application: IHC
Impact Factor: 6.90
Species: Mouse
Huanglian Jiedu Decoction improves the"central-peripheral"inflammatory microenvironment and enhances the cognitive function of APP/PS1 mice by inhibiting the activation of NLRP3 inflammasome mediated by gut microbiota
Published: 2025-08-07
Journal: Chinese Medicine
Application: IHC,WB
Impact Factor: 5.70
Species: Mouse
PMID: 40770806
Antioxidant-Targeting Synergistic Probiotic Delivery System with Improved Probiotics Viability and Intestinal Retention for Ulcerative Colitis Therapy
Published: 2025-07-31
Journal: ACS Applied Polymer Materials
Application: IF
Impact Factor: 4.70
Species: Mouse
Investigating the protective role of Astragalus polysaccharides against fluoride-induced testicular injury via network pharmacology, molecular docking, and in vivo experiments
Published: 2025-07-25
Journal: REPRODUCTIVE TOXICOLOGY
Application: IHC
Impact Factor: 2.80
Species: Rat
PMID: 40716464
miR-142 regulates the function of microvascular endothelial cells through MAT2B
Published: 2025-06-09
Journal: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Application: WB
Impact Factor: 3.00
Species: Rat
PMID: 40499634
Alleviation Effect of the Secondary Metabolite of Anthocyanin (Protocatechuic Acid) on Heterocyclic Amine (IQ)-Induced Liver Injury and Its Underlying Mechanism
Published: 2025-04-08
Journal: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Application: IHC
Impact Factor: 6.20
Species: Mouse
PMID: 40198206
LncRNA FOXD3-AS1 modulates ER stress and epithelial barrier dysfunction in allergic rhinitis by destabilizing CHOP mRNA
Published: 2025-03-11
Journal: CELLULAR SIGNALLING
Application: IHC
Impact Factor: 3.70
Species: Mouse
PMID: 40081548
Using gut microbiota and non-targeted metabolomics techniques to study the effect of xylitol on alleviating DSS-induced inflammatory bowel disease in mice
Published: 2025-03-10
Journal: BMC IMMUNOLOGY
Application: IHC
Impact Factor: 2.70
Species: Mouse
PMID: 40065221
Gut microbiota contribution to selenium deficiency-induced gut–liver inflammation
Published: 2023-09-07
Journal: BIOFACTORS
Application: IHC
Impact Factor: 6.00
Species: Mouse
PMID: 37676478
Kaempferol alleviates acute alcoholic liver injury in mice by regulating intestinal tight junction proteins and butyrate receptors and transporters
Published: 2019-11-27
Journal: TOXICOLOGY
Application: IHC
Impact Factor: 3.55
Species: Mouse
PMID: 31785310
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Product Promise
We promise Anti-Occludin/OCLN Antibody Picoband® performs excellently in relevant applications for ELISA, IP, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01246-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026