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Product Info Summary
| SKU: | A17553 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Olfactory receptor 4K2 OR4K2 Antibody
SKU/Catalog Number
A17553
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Olfactory receptor 4K2 OR4K2 Antibody (Catalog# A17553). Tested in WB, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Olfactory receptor 4K2 OR4K2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17553)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human OR4K2. Immunogen sequence location: 265 - 314
Reactive Species
A17553 is reactive to OR4K2 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
35.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A17553 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
ICC/IF 1:200 - 1:1000.
ELISA: 1:40000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of the lysates from HT-29 cells using OR4K2 antibody.
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Western blot analysis of lysates from Jurkat cells, using OR4K2 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Olfactory receptor 4K2 OR4K2 Antibody (A17553)
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Customer Q&As
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5 Customer Q&As for Anti-Olfactory receptor 4K2 OR4K2 Antibody
Question
I see that the anti-OR4K2 antibody A17553 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-03-24
Question
Is a blocking peptide available for product anti-OR4K2 antibody (A17553)?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
We do provide the blocking peptide for product anti-OR4K2 antibody (A17553). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-12-13
Question
My question regarding product A17553, anti-OR4K2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A17553 anti-OR4K2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-06
Question
We are currently using anti-OR4K2 antibody A17553 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
A. Mitchell
Verified customer
Asked: 2017-06-01
Answer
The anti-OR4K2 antibody (A17553) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-01
Question
Would A17553 anti-OR4K2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Dhar
Verified customer
Asked: 2016-08-16
Answer
As indicated on the product datasheet, A17553 anti-OR4K2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-08-16
