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Bglap antibody (PB9920) performs well in Flow Cytometry, IHC and shows reactivity with Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 20 Publications
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Flow Cytometry analysis of L6 cells using anti-Osteocalcin antibody (PB9920).
Overlay histogram showing L6 cells stained with PB9920 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Osteocalcin Antibody (PB9920,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing L6 cells stained with PB9920 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Osteocalcin Antibody (PB9920,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-Osteocalcin/BGLAP Antibody
Bglap antibody
| Reactive Species | Host | Application |
|---|---|---|
| Rat | Rabbit | Flow Cytometry, IHC |
Tested Applications
Bglap antibody was positively detected by IHC in:
rat tibia tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Bglap antibody was positively detected by FC in:
L6 cell
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-Osteocalcin/BGLAP Antibody
View all BGLAP Antibodies
View all BGLAP Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat Osteocalcin recombinant protein (Position: Y50-V99). Rat Osteocalcin shares 80.9% and 68.9% amino acid (aa) sequence identity with human and mouse Osteocalcin, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Calculated Molecular Weight
10.9 kDa
Background of Bglap
Osteocalcin, also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP), is a noncollagenous protein found in bone and dentin. In humans, the osteocalcin is encoded by the BGLAP gene. Its receptor is GPRC6A. It is mapped to 1q22. Osteocalcin may play a role in the body's metabolic regulation and is pro-osteoblastic, or bone-building. It acts as a hormone in the body, causing beta cellsin the pancreas to release more insulin, and at the same time directing fat cells to release the hormone adiponectin, which increases sensitivity to insulin. Also, it may play a role in male fertility. And it is found that picomolar amounts of osteocalcin affected insulin secretion and beta-cell proliferation.
Cite This Product
Anti-Osteocalcin/BGLAP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9920)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Rat
100%
High Homology
Sheep
80%
Moderate Homology
Dog
80%
Moderate Homology
Cow
78%
Moderate Homology
Monkey
78%
Moderate Homology
Human
76%
Moderate Homology
Rabbit
72%
Moderate Homology
Mouse
64%
Low homology
Zebrafish
36%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for Bglap antibody
Flow Cytometry (FC) protocol for Bglap antibody
Publications
Application:
Species:
Synergistic effects of erythropoietin and selenium enhance bone regeneration in ovariectomized rats
Published: 2026-06-04
Journal: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
Application: IHC
Impact Factor: 4.70
Species: Rat
PMID: 42240714
An intelligent bacterial capture nanodrug enhances immunotherapy for implant-associated infections by inducing cuproptosis-like death in bacteria
Published: 2025-12-30
Journal: JOURNAL OF CONTROLLED RELEASE
Application: IHC,IF
Impact Factor: 11.50
Species: Rat
PMID: 41478375
Virulence-Selective Trap–Capture–Kill Antibacterial Nanostructures With Immune–Metabolic Regulation for Treating Implant-Associated Infections
Published: 2025-09-15
Journal: Advanced Science
Application: IF,IHC
Impact Factor: 14.10
Species: Rat
PMID: 40952251
Melatonin combined with static magnetic field acquire better protective effects on bone mass and bone strength in osteoporosis induced by ovariectomy in rats
Published: 2025-06-02
Journal: ANNALS OF MEDICINE
Application: IHC
Impact Factor: 4.30
Species: Rat
PMID: 40454678
A photo-triggered bioactive nanohydrogel loaded with niobium carbide for stem cell osteogenic differentiation
Published: 2024-10-28
Journal: Journal of Innovative Optical Health Sciences
Application: IF
Impact Factor: 2.30
Species: Rat
An Integrated Microcurrent Delivery System Facilitates Human Parathyroid Hormone Delivery for Enhancing Osteoanabolic Effect
Published: 2024-10-17
Journal: Small Methods
Application: IHC
Impact Factor: 10.70
Species: Rat
PMID: 39420694
Tauroursodeoxycholic acid combined with selenium accelerates bone regeneration in ovariectomized rats
Published: 2024-10-15
Journal: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
Application: IF
Impact Factor: 4.20
Species: Rat
PMID: 39404912
Cyclosporine a inhibits bone regeneration and induces bone loss in a rat model
Published: 2024-03-29
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: IHC
Impact Factor: 4.80
Species: Rat
PMID: 38552293
Preparation of an injectable and photocurable carboxymethyl cellulose/hydroxyapatite composite and its application in cranial regeneration
Published: 2024-02-27
Journal: CARBOHYDRATE POLYMERS
Application: IHC
Impact Factor: 10.70
Species: Rat
PMID: 38494238
Expression of the HIF-1α/VEGF pathway is upregulated to protect alveolar bone density reduction in nasal-obstructed rats.
Published: 2024-01-02
Journal: HISTOLOGY AND HISTOPATHOLOGY
Application: WB
Impact Factor: 2.50
Species: Rat
PMID: 38235568
Hydroxyapatite-Tethered Peptide Hydrogel Promotes Osteogenesis
Published: 2022-12-08
Journal: Gels
Application: IF
Impact Factor: 4.43
Species: Mouse
PMID: 36547328
β-ecdysone promotes osteogenic differentiation of bone marrow mesenchymal stem cells
Published: 2020-04-30
Journal: JOURNAL OF GENE MEDICINE
Application: IHC
Impact Factor: 3.26
Species: Rat
PMID: 32350960
Surface modification of titanium implants by ZIF-8@Levo/LBL coating for inhibition of bacterial-associated infection and enhancement of in vivo osseointegration
Published: 2020-02-29
Journal: CHEMICAL ENGINEERING JOURNAL
Application: IHC
Impact Factor: 10.65
Species: Rat
Peptide LL-37 coating on micro-structured titanium implants to facilitate bone formation in vivo via mesenchymal stem cell recruitment
Published: 2018-09-25
Journal: Acta Biomaterialia
Application: IHC
Impact Factor: 6.38
Species: Rat
PMID: 30266635
Multilayered coating of titanium implants promotes coupled osteogenesis and angiogenesis in vitro and in vivo
Published: 2018-04-25
Journal: Acta Biomaterialia
Application: IF
Impact Factor: 6.38
Species: Rat,Human
PMID: 29702291
Tim-3 exacerbates kidney ischaemia/reperfusion injury through the TLR-4/NF-κB signalling pathway and an NLR-C4 inflammasome activation
Published: 2018-03-23
Journal: CLINICAL AND EXPERIMENTAL IMMUNOLOGY
Application: IF,IHC
Impact Factor: 3.54
Species: Rat
PMID: 29570768
Functionalizing titanium surface with PAMAM dendrimer and human BMP2 gene via layer-by-layer assembly for enhanced osteogenesis
Published: 2017-10-20
Journal: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Application: IHC
Impact Factor: 3.08
Species: Rat
PMID: 29052311
Demineralized bone matrix used for direct pulp capping in rats
Published: 2017-03-02
Journal: PLoS One
Application: IHC
Impact Factor: 2.81
Species: Rat
PMID: 28253279
IGF1 promotes osteogenic differentiation of mesenchymal stem cells derived from rat bone marrow by increasing TAZ expression
Published: 2013-03-05
Journal: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Application: WB
Impact Factor: 2.41
Species: Rat
PMID: 23473758
Effects of extracts of oxyntic mucosa in rat on the biological activity of osteoblasts
Published: 2009-04-15
Journal: OSTEOPOROSIS INTERNATIONAL
Application: WB
Impact Factor: 4.29
Species: Rat
PMID: 19367351
Review
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1 Review For Anti-Osteocalcin/BGLAP Antibody
PB9920 successfully extracted rat osteocalcin from rat EDTA plasma--Chad Borges, Arizona State University, Biodesign Virginia G. Piper Center for Personalized Diagnostics, Assistant Professor
Source: Customer Feedback Submission
| SKU | PB9920 |
|---|---|
| Applications | Picomole scale immunoprecipitation |
| Sample | Rat EDTA plasma |
| Detection | Bruker maXis 4G mass spectrometer (LC-MS; Q-TOF) |
"The immobilized antibody successfully extracted rat osteocalcin from rat EDTA plasma. The results shown align very well with mass and known posttranslational modifications of rat osteocalcin."
Chad Borges
Verified customer
Submitted 2017-08-21
Product Promise
We promise Anti-Osteocalcin/BGLAP Antibody performs excellently in relevant applications for Flow Cytometry, IHC. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PB9920
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026