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Product Info Summary
|Application:||Flow Cytometry, IHC, ICC|
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Anti-Osteocalcin/BGLAP Antibody Picoband™
Boster Bio Anti-Osteocalcin/BGLAP Antibody Picoband™ catalog # PB9920. Tested in Flow Cytometry, IHC, ICC applications. This antibody reacts with Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Osteocalcin/BGLAP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9920)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived rat Osteocalcin recombinant protein (Position: Y50-V99). Rat Osteocalcin shares 80.9% and 68.9% amino acid (aa) sequence identity with human and mouse Osteocalcin, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
PB9920 is reactive to Bglap in Rat
PB9920 is guaranteed for Flow Cytometry, IHC, ICC Boster Guarantee
Background of Osteocalcin
Osteocalcin, also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP), is a noncollagenous protein found in bone and dentin. In humans, the osteocalcin is encoded by the BGLAP gene. Its receptor is GPRC6A. It is mapped to 1q22. Osteocalcin may play a role in the body's metabolic regulation and is pro-osteoblastic, or bone-building. It acts as a hormone in the body, causing beta cellsin the pancreas to release more insulin, and at the same time directing fat cells to release the hormone adiponectin, which increases sensitivity to insulin. Also, it may play a role in male fertility. And it is found that picomolar amounts of osteocalcin affected insulin secretion and beta-cell proliferation.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat
Immunocytochemistry, 0.5-1μg/ml, Rat
Flow Cytometry, 1-3μg/1x106 cells, Rat
Validation Images & Assay Conditions
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Figure 1. IHC analysis of Osteocalcin using anti-Osteocalcin antibody (PB9920).
Osteocalcin was detected in paraffin-embedded section of rat tibia tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Osteocalcin Antibody (PB9920) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 2. Flow Cytometry analysis of L6 cells using anti-Osteocalcin antibody (PB9920).
Overlay histogram showing L6 cells stained with PB9920 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Osteocalcin Antibody (PB9920,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Gene/Protein Information For Bglap (Source: Uniprot.org, NCBI)
osteocalcin/matrix Gla protein family
BGLAP; BGP; bone gamma-carboxyglutamate (gla) protein (osteocalcin); bone gamma-carboxyglutamate (gla) protein; Bone Gla protein; Gamma-carboxyglutamic acid-containing protein; OC; OCN; Osteocalcin Bglap|Bglap2, Bgp, Bgpr, Bgpra|bone gamma-carboxyglutamate protein|osteocalcin|bone Gla-protein|bone gamma-carboxyglutamate (gla) protein (osteocalcin)|bone gamma-carboxyglutamate protein 2|bone gamma-carboxyglutamic acid (Gla) protein (osteocalcin)|gamma-carboxyglutamic acid-containing protein*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on Bglap, check out the Bglap Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Bglap: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
PB9920 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Caiyun Mu,Ye He,Yan Hu,Menghuan Li,Maowen Chen,Rong Wang,Yang Xiang, Zhong Luo,Kaiyong Cai,Construction of chemokine substance P-embedded biomimetic multilayer onto bioactive magnesium silicate-titanium implant for bone regeneration,Applied Materials Toda
PB9920 usage in article: APP:WB, SAMPLE:MSCS, DILUTION:NA
Intramuscular injection of bone marrow mononuclear cells contributes to bone repair following midpalatal expansion in rats.
The Osteogenic Potential of Mesoporous Bioglasses/Silk and Non-Mesoporous Bioglasses/Silk Scaffolds in Ovariectomized Rats: In vitro and In vivo Evaluation
Synergistic effects of fibroblast growth factor-2 and bone morphogenetic protein-2 on bone induction
Role of glucocorticoid-induced leucine zipper (GILZ) in inflammatory bone loss
Effects of Naringin on Proliferation and Osteogenic Differentiation of Human Periodontal Ligament Stem Cells In Vitro and In Vivo
Electrospun Poly(L-lactide)/Poly(?-caprolactone) Blend Nanofibrous Scaffold: Characterization and Biocompatibility with Human Adipose-Derived Stem Cells
Liu Q, Ma Y, Wang J, Zhu X, Yang Y, Mei Y. PLoS One. 2017 Mar 2;12(3):e0172693. doi: 10.1371/journal.pone.0172693. eCollection 2017. Demineralized bone matrix used for direct pulp capping in rats
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