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Bglap antibody (PB9919) performs well in IHC and shows reactivity with Mouse.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 16 Publications

pb9919 bglap primary antibodies ihc testing 1

IHC analysis of Osteocalcin/BGLAP using anti-Osteocalcin/BGLAP antibody (PB9919).
Osteocalcin/BGLAP was detected in a paraffin-embedded section of mouse tibia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Osteocalcin/BGLAP Antibody (PB9919) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Anti-Osteocalcin/BGLAP Antibody

Bglap antibody

16 Publications

Reactive Species	Host	Application
Mouse	Rabbit	IHC

Tested Applications

Bglap antibody was positively detected by IHC in:

mouse tibia tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Mouse

Tested

Recommend

Product Information

Product Name

Anti-Osteocalcin/BGLAP Antibody
View all BGLAP Antibodies

Form

Lyophilized

Uniprot

P86546

Gene ID

12096

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse Osteocalcin recombinant protein (Position: Y50-I95). Mouse Osteocalcin shares 69% and 68.9% amino acid (aa) sequence identity with human and rat Osteocalcin, respectively.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Calculated Molecular Weight

10.5 kDa

Background of Bglap

Osteocalcin, also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP), is a noncollagenous protein found in bone and dentin. In humans, the osteocalcin is encoded by the BGLAP gene. Its receptor is GPRC6A. It is mapped to 1q22. Osteocalcin may play a role in the body's metabolic regulation and is pro-osteoblastic, or bone-building. It acts as a hormone in the body, causing beta cellsin the pancreas to release more insulin, and at the same time directing fat cells to release the hormone adiponectin, which increases sensitivity to insulin. Also, it may play a role in male fertility. And it is found that picomolar amounts of osteocalcin affected insulin secretion and beta-cell proliferation.

Cite This Product

Anti-Osteocalcin/BGLAP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9919)

Protocols

Immunohistochemistry (IHC) protocol for Bglap antibody

Review Protocol

Publications

Chitosan‑selenium nanoparticles mitigate fluoride-induced bone injury by regulating OPG/RANKL/NF-κB pathway through the gut-bone axis

Published: 2026-06-23

Journal: FOOD RESEARCH INTERNATIONAL

Application: IHC

Impact Factor: 8.80

Species: Mouse

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Anti-Osteocalcin/BGLAP Antibody

Bglap antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Chitosan‑selenium nanoparticles mitigate fluoride-induced bone injury by regulating OPG/RANKL/NF-κB pathway through the gut-bone axis

Light-Switched Mesenchymal Stem Cells for In Situ Exosome Amplification in Craniofacial Bone Defect Reconstruction

A Novel Nonsense Variant in TBX15 Transcription Factor Suggests an Expanded Genetic Spectrum Of Submucous Cleft Palate

Preparation, Physicochemical Characterization, and In Vitro and In Vivo Osteogenic Evaluation of Reinforced PLLA-PLCL/HA Resorbable Membranes

Alterations of gut virome with close interaction in the progression of estrogen deficiency-induced osteoporosis

Histopathological Characterization of Mandibular Condyles in Four Temporomandibular Joint Osteoarthritis Mouse Models

Porous Nano-Fiber Structure of Modified Electrospun Chitosan GBR Membranes Improve Osteoblast Calcium Phosphate Deposition in Osteoblast-Fibroblast Co-Cultures

Regulatory T cell-derived exosome mediated macrophages polarization for osteogenic differentiation in fracture repair

The Critical Role of The Piezo1/β-catenin/ATF4 Axis on The Stemness of Gli1+ BMSCs During Simulated Microgravity-Induced Bone Loss

Spatial and Temporal Expression of Ectodysplasin-A Signaling Pathway Members During Mandibular Condylar Development in Postnatal Mice

Mechanosensitive Piezo1 is crucial for periosteal stem cell-mediated fracture healing

Identification of Dental Stem Cells Similar to Skeletal Stem Cells

Sal B targets TAZ to facilitate osteogenesis and reduce adipogenesis through MEK-ERK pathway

Role of glucocorticoid-induced leucine zipper (GILZ) in inflammatory bone loss

Dentin Sialophosphoprotein-Promoted Mineralization and Expression of Odontogenic Genes in Adipose-Derived Stromal Cells

[Effects of secretive bone morphogenetic protein 2 induced by gene transfection on the biological changes of NIH3T3 cells].

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