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- Table of Contents
P2RX7 antibody (A01208-3) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Flow Cytometry analysis of THP-1 cells using anti-P2X7/P2RX7 antibody (A01208-3).
Overlay histogram showing THP-1 cells stained with A01208-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-P2X7/P2RX7 Antibody (A01208-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing THP-1 cells stained with A01208-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-P2X7/P2RX7 Antibody (A01208-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-P2X7/P2RX7 Antibody Picoband®
P2RX7 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
P2RX7 antibody was positively detected by WB in:
rat brain tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
P2RX7 antibody was positively detected by IHC in:
human liver cancer tissue, human gall bladder adenosquamous carcinomar tissue, rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
P2RX7 antibody was positively detected by FC in:
THP-1 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human, Rat
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Human
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-P2X7/P2RX7 Antibody Picoband®
View all P2RX7 Antibodies
View all P2RX7 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human P2X7/P2RX7 recombinant protein (Position: S47-K311).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
70 kDa
Calculated Molecular Weight
68.6 kDa
Background of P2RX7
P2X purinoceptor 7 is a protein that in humans is encoded by the P2RX7 gene. The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria.
Cite This Product
Anti-P2X7/P2RX7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01208-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Rabbit
93%
High Homology
Dog
89%
Moderate Homology
Sheep
86%
Moderate Homology
Mouse
81%
Moderate Homology
Rat
80%
Moderate Homology
Zebrafish
53%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for P2RX7 antibody
Immunohistochemistry (IHC) protocol for P2RX7 antibody
Flow Cytometry (FC) protocol for P2RX7 antibody
Publications
Application:
Species:
P2X7 receptor antagonists modulate experimental autoimmune neuritis via regulation of NLRP3 inflammasome activation and Th17 and Th1 cell differentiation
Published: 2024-03-25
Journal: Journal of Neuroinflammation
Application: FC
Impact Factor: 9.30
Species: Human,Rat
PMID: 38528529
LncTCONS_00058568 is involved in the pathophysiologic processes mediated by P2X7R in the lower thoracic spinal cord after acute kidney injury
Published: 2024-03-18
Journal: FASEB JOURNAL
Application: IHC,IF,WB
Impact Factor: 4.40
Species: Rat
PMID: 38498358
Increased expression of P2X7 receptor in peripheral blood mononuclear cells correlates with clinical severity and serum levels of Th17-related cytokines in patients with myasthenia gravis
Published: 2017-04-20
Journal: CLINICAL NEUROLOGY AND NEUROSURGERY
Application: WB
Impact Factor: 1.38
Species: Human
PMID: 28458152
Review
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1 Review For Anti-P2X7/P2RX7 Antibody Picoband®
This antibody is highly specific and efficient, suitable for Western blot detection of P2RX7 protein in HT22 cells, producing clear and single bands.
| SKU | A01208-3 |
|---|---|
| Application | Western Blot |
| Sample | Mouse HT-22 cells |
| Sample Processing Description | After digestion, cells were collected by centrifugation and lysed in 1 mL of RIPA buffer containing protease inhibitors on ice for 1 h. The lysates were centrifuged, and the supernatants were collected. After protein quantification, 5× loading buffer was added, and the samples were denatured at 100 °C for 10 min before loading onto SDS-PAGE. |
| Other Reagents | Blocking buffer |
| Primary Antibody | P2X7/P2RX7 Antibody Picoband® |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) |
| Secondary Incubation | 1:2000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | The control represents HT22 cells without drug treatment, while Low, Medium, and High correspond to experimental groups treated with three different drug concentrations for 24 h. The Western blot results obtained with this antibody are clear and well-defined, allowing comparison of expression differences between groups. The expression of the target protein slightly increases with increasing drug concentration. |
Yu Tian, Institute of Materia Medica, Beijing
Verified customer
Submitted 2025-12-26
Product Promise
We promise Anti-P2X7/P2RX7 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A01208-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026