Anti-P70 S6 Kinase RPS6KB1 Monoclonal Antibody

Product Name

Anti-P70 S6 Kinase RPS6KB1 Monoclonal Antibody

View all p70 S6 Kinase/S6K Antibodies

SKU/Catalog Number

M01475S6

Size

100ul

Form

Liquid

Description

Boster Bio Anti-P70 S6 Kinase RPS6KB1 Monoclonal Antibody catalog # M01475S6. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-P70 S6 Kinase RPS6KB1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01475S6)

Host

Mouse

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Monoclonal

Clone Number

1C7

Isotype

IgG

Immunogen

Recombinant peptide derived from P70 S6 Kinase

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

M01475S6 is reactive to RPS6KB1 in Human, Mouse, Rat

Applications

M01475S6 is guaranteed for IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

59.14kDa

Background of p70 S6 Kinase/S6K

TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IHC 1:100-200

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Immunohistochemistry validation of RPS6KB1 using Anti-P70 S6 Kinase RPS6KB1 Monoclonal Antibody (M01475S6).

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Lung Carcinoma Tissue using P70 S6 Kinase Mouse Monoclonal Antibody diluted at 1:200.
For more protocol information of IHC

Click image to see more details

Figure 2. Immunohistochemistry validation of RPS6KB1 using Anti-P70 S6 Kinase RPS6KB1 Monoclonal Antibody (M01475S6).

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Breast Carcinoma Tissue using P70 S6 Kinase Mouse Monoclonal Antibody diluted at 1:200.
For more protocol information of IHC

Gene/Protein Information For RPS6KB1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.11; EC 2.7.11.1; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6 Kinase; p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1; p70 S6 kinase, alpha 2; p70 S6KA; p70 S6K-alpha; p70(S6K)-alpha; p70-alpha; p70-S6K; P70S6K1; PS6K; ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kD, polypeptide 1; ribosomal protein S6 kinase, 70kDa, polypeptide 1; RPS6KB1; S6K; S6K1; S6K1p70-S6K 1; S6K-beta-1; serine/threonine kinase 14 alpha; Serine/threonine-protein kinase 14A; STK14A RPS6KB1 PS6K, S6K, S6K-beta-1, S6K1, STK14A, p70 S6KA, p70(S6K)-alpha, p70-S6K, p70-alpha ribosomal protein S6 kinase B1 ribosomal protein S6 kinase beta-1|ribosomal protein S6 kinase I|ribosomal protein S6 kinase, 70kDa, polypeptide 1|serine/threonine kinase 14 alpha|serine/threonine-protein kinase 14A

For more info on RPS6KB1, check out the RPS6KB1 Infographic

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In this infographic, you will see the following information for RPS6KB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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