Click image to see more details
Product Info Summary
| SKU: | P05832 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho Cyclin C (Ser275) CCNC Antibody
SKU/Catalog Number
P05832
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho Cyclin C (Ser275) CCNC Antibody (Catalog# P05832). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho Cyclin C (Ser275) CCNC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P05832)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human Cyclin C around the phosphorylation site of Ser275. Immunogen sequence location: 234-283
Reactive Species
P05832 is reactive to CCNC in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
33.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P05832 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:10000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Cyclin C (Phospho-Ser275) Antibody
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Cyclin C (Phospho-Ser275) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from NIH/3T3 cells treated with UV 15', using Cyclin C (Phospho-Ser275) Antibody. The lane on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho Cyclin C (Ser275) CCNC Antibody (P05832)
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Customer Q&As
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4 Customer Q&As for Anti-Phospho Cyclin C (Ser275) CCNC Antibody
Question
I see that the anti-Phospho Cyclin C (Ser275) antibody P05832 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-02-13
Question
We are currently using anti-Phospho Cyclin C (Ser275) antibody P05832 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
The anti-Phospho Cyclin C (Ser275) antibody (P05832) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-22
Question
I have a question about product P05832, anti-Phospho Cyclin C (Ser275) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P05832 anti-Phospho Cyclin C (Ser275) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-01
Question
Would P05832 anti-Phospho Cyclin C (Ser275) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
P. Gonzalez
Verified customer
Asked: 2016-02-19
Answer
It shows on the product datasheet, P05832 anti-Phospho Cyclin C (Ser275) antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-02-19
