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Product Info Summary
| SKU: | P00023-5 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Phospho-EGFR (Y1173) Rabbit Monoclonal Antibody, Clone#RM269
SKU/Catalog Number
P00023-5
Size
100uL
Form
Liquid
Description
Boster Bio Anti-Phospho-EGFR (Y1173) Rabbit Monoclonal Antibody, Clone#RM269 (Catalog # P00023-5). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-EGFR (Y1173) Rabbit Monoclonal Antibody, Clone#RM269 (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00023-5)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM269
Isotype
Rabbit IgG
Immunogen
A phospho-peptide corresponding to human Phospho- EGFR (Tyr1173)
Cross-reactivity
This antibody reacts to human EGF Receptor only when phosphorylated at Tyr1173. There is no cross-reactivity to EGFR without phosphorylation at Tyr1173.
Reactive Species
P00023-5 is reactive to EGFR in Human
Calculated molecular weight
134.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00023-5 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:1000-1:5000 dilution.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of A431 cell nontreated (-) or treated (+) with EGF, using Anti-Phospho-EGFR (Tyr1173) Rabbit Monoclonal Antibody (clone RM269) at a 1:500 dilution, showed Phospho-EGFR (Tyr1173) in EGF-treated A431 cells.
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IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Phospho-EGFR (Tyr1173) Rabbit Monoclonal Antibody (clone RM269) at a 1:5000 dilution.
Specific Publications For Anti-Phospho-EGFR (Y1173) Rabbit Monoclonal Antibody, Clone#RM269 (P00023-5)
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