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Product Info Summary
| SKU: | MP04922 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-MSK1 (S376) RPS6KA5 Monoclonal Antibody
SKU/Catalog Number
MP04922
BM4502 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-MSK1 (S376) RPS6KA5 Monoclonal Antibody catalog # MP04922. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-MSK1 (S376) RPS6KA5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP04922)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FCF-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-MSK1 (S376)
Reactive Species
MP04922 is reactive to RPS6KA5 in Human, Rat
Observed Molecular Weight
80, 90 kDa
Calculated molecular weight
89.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MP04922 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: HEK293 cell
IHC: human lung cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of Phospho-MSK1 (S376) expression in HEK293 cell lysate treated with EGF.
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Immunohistochemical analysis of paraffin-embedded human lung cancer, using Phospho-MSK1 (S376) Antibody.
Specific Publications For Anti-Phospho-MSK1 (S376) RPS6KA5 Monoclonal Antibody (MP04922)
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Customer Q&As
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8 Customer Q&As for Anti-Phospho-MSK1 (S376) RPS6KA5 Monoclonal Antibody
Question
Could you confirm the IHC image sample with Abways for Anti-Phospho-MSK1 (S376) Monoclonal Antibody MP04922 | CY5663 ? The customer said it looks like brain, but the abways website says "Immunohistochemical analysis of paraffin-embedded human lung cancer, using Phospho-MSK1 (S376) Antibody."
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
It is brain. We are going to remove the image and upload the new one after receiving it from Abways.
Boster Scientific Support
Answered: 2020-03-13
Question
Does anti-Phospho-MSK1 (S376) Monoclonal antibody MP04922 work on horse IHC with caudate nucleus?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
Our lab technicians have not validated anti-Phospho-MSK1 (S376) Monoclonal antibody MP04922 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Phospho-MSK1 (S376) Monoclonal antibody MP04922 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse caudate nucleus in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-21
Question
Here is the WB image, lot number and protocol we used for pancreas {eco:0000269 using anti-Phospho-MSK1 (S376) Monoclonal antibody MP04922. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-30
Question
I was wanting to use your anti-Phospho-MSK1 (S376) Monoclonal antibody for WB for human pancreas eco:0000269 on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pancreas {eco:0000269 identification?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
It shows on the product datasheet, MP04922 anti-Phospho-MSK1 (S376) Monoclonal antibody has been validated for IP, IF, IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human pancreas {eco:0000269 in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-29
Question
I see that the anti-Phospho-MSK1 (S376) Monoclonal antibody MP04922 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-05-28
Question
Does anti-Phospho-MSK1 (S376) Monoclonal antibody MP04922 work for WB with pancreas {eco:0000269?
Verified Customer
Verified customer
Asked: 2019-02-12
Answer
According to the expression profile of pancreas {eco:0000269, RPS6KA5 is highly expressed in pancreas {eco:0000269. So, it is likely that anti-Phospho-MSK1 (S376) Monoclonal antibody MP04922 will work for WB with pancreas {eco:0000269.
Boster Scientific Support
Answered: 2019-02-12
Question
Do you have a BSA free version of anti-Phospho-MSK1 (S376) Monoclonal antibody MP04922 available?
S. Patel
Verified customer
Asked: 2017-12-11
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-MSK1 (S376) Monoclonal antibody MP04922 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-12-11
Question
Will MP04922 anti-Phospho-MSK1 (S376) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
N. Thomas
Verified customer
Asked: 2015-06-11
Answer
It shows on the product datasheet, MP04922 anti-Phospho-MSK1 (S376) Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-06-11
