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Product Info Summary
| SKU: | P00096 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Phospho-PDGF Receptor beta (Y740) PDGFRB Rabbit Monoclonal Antibody
SKU/Catalog Number
P00096
BM4512 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-PDGF Receptor beta (Y740) PDGFRB Rabbit Monoclonal Antibody catalog # P00096. Tested in WB, ICC/IF applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-PDGF Receptor beta (Y740) PDGFRB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00096)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FDF-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-PDGF Receptor beta (Y740)
Reactive Species
P00096 is reactive to PDGFRB in Mouse, Rat
Observed Molecular Weight
190 kDa
Calculated molecular weight
124.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00096 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
Positive Control
WB: NIH 3T3
Validation Images & Assay Conditions
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Western blot analysis of Phospho-PDGF Receptor beta (Y740) expression in NIH 3T3 treated with PDGF.
Specific Publications For Anti-Phospho-PDGF Receptor beta (Y740) PDGFRB Rabbit Monoclonal Antibody (P00096)
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Customer Q&As
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4 Customer Q&As for Anti-Phospho-PDGF Receptor beta (Y740) PDGFRB Rabbit Monoclonal Antibody
Question
We have seen staining in mouse brain. Any tips? Is anti-Phospho-PDGF Receptor beta (Y740) Rabbit Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
According to literature brain does express PDGFRB. According to Uniprot.org, PDGFRB is expressed in ectocervix, brain, among other tissues. Regarding which tissues have PDGFRB expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-11-08
Question
We are currently using anti-Phospho-PDGF Receptor beta (Y740) Rabbit Monoclonal antibody P00096 for mouse tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
The anti-Phospho-PDGF Receptor beta (Y740) Rabbit Monoclonal antibody (P00096) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-29
Question
My colleagues were satisfied with the WB result of your anti-Phospho-PDGF Receptor beta (Y740) Rabbit Monoclonal antibody. However we have seen positive staining in ectocervix cell membrane using this antibody. Is that expected? Could you tell me where is PDGFRB supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
Based on literature, ectocervix does express PDGFRB. Generally PDGFRB expresses in cell membrane. Regarding which tissues have PDGFRB expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-05-29
Question
Our lab used your anti-Phospho-PDGF Receptor beta (Y740) Rabbit Monoclonal antibody for IF on ectocervix in a previous project. I am using rat, and I plan to use the antibody for WB next. We are interested in examining ectocervix as well as brain in our next experiment. Could give a recommendation on which antibody would work the best for WB?
C. Jha
Verified customer
Asked: 2013-08-23
Answer
I viewed the website and datasheets of our anti-Phospho-PDGF Receptor beta (Y740) Rabbit Monoclonal antibody and it appears that P00096 has been validated on rat in both IF and WB. Thus P00096 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-08-23
