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STAT3 antibody (P00007-2) performs well in IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 41 Publications

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Immunofluorescent analysis of HeLa cells treated with IFN-alpha, using Phospho-STAT3 (Y705) Antibody.

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Anti-Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody

Name: Anti-Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody
Brand: Boster Bio
SKU: P00007-2
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

STAT3 antibody

41 Publications|

1 Review

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Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	IP, IF, IHC, ICC, WB

Tested Applications

STAT3 antibody was positively detected by WB in:

human Hela whole cell, mouse NIH/3T3 whole cell, mouse RAW264.7 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

STAT3 antibody was positively detected by IHC in:

human lung cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

STAT3 antibody was positively detected by ICC/IF in:

HeLa cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Mouse

Tested

Recommend

Rat

Recommend

Product Information

Product Name

Anti-Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody
View all STAT3 Antibodies

Form

Liquid

Uniprot

P40763

Gene ID

6774

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

IFA-19

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human STAT3

Observed Molecular Weight

88 kDa

Calculated Molecular Weight

88.1 kDa

Cite This Product

Anti-Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00007-2)

Protocols

Western Blot protocol for STAT3 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for STAT3 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for STAT3 antibody

Review Protocol

Publications

HACD3 Integrates Central Leptin Sensitivity and Peripheral Thermogenesis to Oppositely Regulate Energy Balance

Published: 2026-08-22

Journal: JOURNAL OF NUTRITIONAL BIOCHEMISTRY

Application: WB

Impact Factor: 5.20

Species: Mouse

PMID: 42628587

SKU	P00007-2
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents	Blocking buffer
Primary Antibody	Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1:5000, 1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The figure shows representative Western blot results of the target protein STAT3 (Phospho-Y705) and the internal control Actin in rat colon tissue from the normal control group, disease model group, low-, medium-, and high-dose traditional Chinese medicine–treated groups, and the western medicine–treated group. The target bands are clear and well defined, and the experimental results are satisfactory.

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Anti-Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody

STAT3 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

HACD3 Integrates Central Leptin Sensitivity and Peripheral Thermogenesis to Oppositely Regulate Energy Balance

IDO1 Inhibitor RY103 Prevents Sepsis-Associated Immunosuppression in Cecal Ligation and Puncture Mice

Discovery of Deuterated 3,4-Dihydro-benzo[e][1,4]diazepine-2,5-dione Derivatives as Potent, Selective and Orally Bioavailable ROCK2 Inhibitors

Cyclopeptide heterophyllin from Pseudostellaria heterophylla alleviates neuroinflammation: a study integrating molecular dynamics, inflammatory pathway analysis, and in vivo validation

S-equol promotes ferroptosis in triple negative breast cancer by coordinating NCOA4-mediated ferritinophagy and PPARγ-mediated lipid metabolism

FXR-mediated transcriptional regulation of KLF11 mitigates contrast-induced acute kidney injury via suppressing JAK2/STAT3 pathway

Cadherin-26 contributes to an M2-like macrophage polarization and TGF-β1 expression via the CTNNB1-STAT3 axis in interstitial lung disease

Synergistic Antitumor Activity and Neuroprotective Effects of FGF1/FGFR Inhibition with Oxaliplatin Chemotherapy

M2 macrophage-derived exosomes mitigate acute inflammation following ischemic stroke

Synergistic effects of diammonium glycyrrhizinate and Vitamin D3 against EMT in pulmonary fibrosis through modulating the crosstalk of STAT3/HSP90AA1 and HIF-1α pathway

Pharmacodynamic substances and molecular mechanisms of Tsantan Sumtang alleviating hypoxic pulmonary vascular remodeling

Engineered Panax notoginseng polysaccharide micelles inhibit macrophage polarization and delay the progression of rheumatoid arthritis via JAK2-STAT3 signaling pathway

Reversal of sorafenib resistance in hepatocellular carcinoma by curcumol: insights from network pharmacology, molecular docking, and experimental validation

Sappanone A attenuates rheumatoid arthritis via inhibiting PI3K/AKT/NF-κB and JAK2/STAT3 signaling pathways in vivo and in vitro

In situ formed reactive oxygen species-responsive dipyridamole prodrug hydrogel: Spatiotemporal drug delivery for chemoimmunotherapy

Inhibition of JAK/STAT3 Expression by Acute Myeloid Leukemia-Targeted Nanoliposome for Chemotherapy Enhancement

Irisin alleviates the pyroptosis of β cells in T2DM by inhibiting NLRP3 inflammasome through regulating miR-19b-3p/SOCS3/STAT3 axis mediated autophagy

Piperlongumine inhibits hepatocellular carcinoma growth by modulating GSTP1 and restraining the STAT3 signaling pathway

α-linolenic acid ameliorates pentylenetetrazol-induced neuron apoptosis and neurological impairment in mice with seizures via down-regulating JAK2/STAT3 pathway

Somatic PDGFRB activating variants promote smooth muscle cell phenotype modulation in intracranial fusiform aneurysm

PCSK5 downregulation promotes the inhibitory effect of andrographolide on glioblastoma through regulating STAT3

The direct impact of pegvisomant on osteoblast functions and bone development

Modified Biejia Jianwan decoction restrains PD-L1-mediated immune evasion through the HIF-1α/STAT3/NF-κB signaling pathway

Pancreatic neuroendocrine tumor progression and resistance to everolimus: the crucial role of NF-kB and STAT3 interplay

Integration analysis using bioinformatics and experimental validation on the clinical and biological significance of TSLP in cancers

(2E)-1-(2,4,6-Trimethoxyphenyl)-3-(4-chlorophenyl)prop-2-en-1-one, a Chalcone Derivative, Promotes Apoptosis by Suppressing RAS-ERK and AKT/FOXO3a Pathways in Hepatocellular Carcinoma Cells

The Large Molecular Weight Polysaccharide from Wild Cordyceps and Its Antitumor Activity on H22 Tumor-Bearing Mice

The expression of Hexokinase 2 and its hub genes are correlated with the prognosis in glioma

Curcumin and its nano-formulations: Defining triple-negative breast cancer targets through network pharmacology, molecular docking, and experimental verification

Overexpression of miR-125a-5p Inhibits Hepatocyte Proliferation through the STAT3 Regulation In Vivo and In Vitro

miR-21, miR-125b, and let-7b contribute to the involution of atretic follicles and corpus lutea in Tibetan sheep ovaries

Triptolide attenuates LPS-induced activation of RAW 264.7 macrophages by inducing M1-to-M2 repolarization via the mTOR/STAT3 signaling

Cryptotanshinone ameliorates MPP+-induced oxidative stress and apoptosis of SH-SY5Y neuroblastoma cells: the role of STAT3 in Parkinson’s disease

Folic acid intervention changes liver Foxp3 methylation and ameliorates the damage caused by Th17/Treg imbalance after long-term alcohol exposure

Water extract of sporoderm-broken spores of Ganoderma lucidum enhanced pd-l1 antibody efficiency through downregulation and relieved complications of pd-l1 monoclonal antibody

JAK2/STAT3 regulates estrogen-related senescence of bone marrow stem cells

JAK2-STAT3 signaling pathway is involved in rat periapical lesions induced by Enterococcus faecalis

Glycosylation of dentin matrix protein 1 is critical for fracture healing via promoting chondrogenesis

ELP2 negatively regulates osteoblastic differentiation impaired by tumor necrosis factor α in MC3T3–E1 cells through STAT3 activation

Prognostic role of STAT3 in solid tumors: a systematic review and meta-analysis

Expression and clinical significance of STAT3, P-STAT3, and VEGF-C in small cell lung cancer.

Review

Have you used Anti-Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody?

1 Review For Anti-Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody

This antibody is highly efficient and specific, suitable for Western blot detection of STAT3 (Phospho-Y705) protein in rat colon tissue, with only minor nonspecific bands observed.

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