Anti-Phospho-TBC1D4 (S318) Antibody

Product Name

Anti-Phospho-TBC1D4 (S318) Antibody

View all TBC1D4 Antibodies

SKU/Catalog Number

P02004

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-Phospho-TBC1D4 (S318) Antibody catalog # P02004. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

Cite This Product

Anti-Phospho-TBC1D4 (S318) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02004)

Host

Rabbit

Contents

500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Immunogen

A synthesized peptide derived from human AS160 around the phosphorylation site of Ser318.

Reactive Species

P02004 is reactive to TBC1D4 in Human, Mouse, Rat

Calculated molecular weight

146.6 kDa

Background of TBC1D4

AS160?(Akt substrate of 160 kDa), which was originally known as?TBC1 domain family member 4?(TBC1D4),?is a Rab?GTPase-activating?protein?that in humans is encoded by the?TBC1D4?gene. This gene is a member of the Tre-2/BUB2/CDC16 domain family. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

P02004 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 1:500-2000
Immunohistochemistry, 1:50-200

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

See full target information TBC1D4

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