Anti-PLK1 (Phospho-T210) Antibody (Monoclonal, 32P10)

Product Name

Anti-PLK1 (Phospho-T210) Antibody (Monoclonal, 32P10)

View all PLK1 Antibodies

SKU/Catalog Number

M00182-3

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-PLK1 (Phospho-T210) Antibody (Monoclonal, 32P10) catalog # M00182-3. Tested in WB, IHC, IF, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PLK1 (Phospho-T210) Antibody (Monoclonal, 32P10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00182-3)

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

32P10

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Thr210 of Human PLK1 aa 183-228.

Reactive Species

M00182-3 is reactive to PLK1 in Human, Mouse

Calculated molecular weight

68.3 kDa

Background of PLK1

Serine/threonine-protein kinase PLK1, also known as polo-like kinase 1 (PLK-1) or serine/threonine-protein kinase 13(STPK13), is an enzyme that in humans is encoded by the PLK1 (polo-like kinase 1) gene. Plk1 is an early trigger for G2/M transition. It supports the functional maturation of the centrosome in late G2/early prophaseand establishment of the bipolar spindle. Also, Plk1 phosphorylates and activates cdc25C, a phosphatase that dephosphorylates and activates the cyclinB/cdc2 complex. Studies have shown that the loss of PLK1 expression can inducepro-apoptotic pathways and inhibit growth. Based on yeast and murine studies of meiosis, human PLK1 may also have a regulatory function in meiosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M00182-3 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 1:500-2000
Immunohistochemistry, 1:50-200
Immunofluorescence, 1:50-200
Immunocytochemistry/Immunofluorescence, 1:50-200
Flow Cytometry (Fixed), 1:50-200

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

See full target information PLK1

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis