|Product Name||Anti-PMVK Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for Phosphomevalonate kinase(PMVK) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-PMVK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1067)|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human PMVK(135-151aa EQSRQQRGWVFTPGVDD), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Images And Assay Conditions
Anti-PMVK antibody, PA1067, Western blotting
WB: Rat Cardiac Muscle Tissue Lysate
Anti-PMVK antibody, PA1067, IHC(P)
IHC(P): Rat Skeletal Muscle Tissue
Protein Target Info (Source: Uniprot.org)
|Protein Name||Phosphomevalonate kinase|
|Tissue Specificity||Heart, liver, skeletal muscle, kidney, and pancreas. Lower level in brain, placenta and lung. .|
|Alternative Names||Phosphomevalonate kinase;PMKase;hPMK;22.214.171.124;PMVK;PMKI;|
|Subcellular Localization||Peroxisome .|
|Molecular Weight||21995 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Research Areas||Cancer, Cancer Metabolism, Lipid And Lipoprotein Metabolism, Lipid Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Lipids And Lipoproteins, Pathways And Processes, Signal Transduction
*You can search these to find other products in these research areas.
|Background||PMVK(phosphomevalonate kinase) is a cytosolic enzyme that catalyzes the conversion of mevalonate-5-phosphate to mevalonate-5-diphosphate. It is mapped to chromosome 1p13-1q22-23 and spans more than 8.4 kb in the human genome. PMVK is a peroxisomal protein which requires the C-terminal peroxisomal targeting signal, Ser-Arg-Leu, for localization to the organelle. It was expressed highly in heart, liver, skeletal muscle, kidney and pancreas and slightly lower in brain, placenta, and lung. And PMKase gene expression is subject to regulation by sterol at the level of transcription. It is a single copy gene covering less than 15 kb in the human genome. The human PMKase amino acid sequence contains a consensus peroxisomal targeting sequence(PTS-1), Ser-Arg-Leu, at the C terminus of the protein.|
Other Recommended Resources
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1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,