Product Info Summary
| SKU: | A02434-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Pre-mRNA-processing factor 19 PRPF19 Antibody
SKU/Catalog Number
A02434-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Pre-mRNA-processing factor 19 PRPF19 Antibody catalog # A02434-1. Tested in WB,IHC,ICC/IF,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Pre-mRNA-processing factor 19 PRPF19 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02434-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human PRP19(NP_055317.1).
Reactive Species
A02434-1 is reactive to PRPF19 in Human, Mouse, Rat
Calculated molecular weight
55.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02434-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts of various cell lines, using PRP19 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.
Click image to see more details
Immunohistochemistry of paraffin-embedded rat liver using PRP19 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Click image to see more details
Immunohistochemistry of paraffin-embedded human lung cancer using PRP19 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Specific Publications For Anti-Pre-mRNA-processing factor 19 PRPF19 Antibody (A02434-1)
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