Product Info Summary
SKU: | M07004-1 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | IHC, WB |
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Product info
Product Name
Anti-PTK9 (TWF1) Mouse Monoclonal Antibody [Clone ID: OTI5C12]
SKU/Catalog Number
M07004-1
Size
100 µl
Description
Boster Bio TWF1 mouse monoclonal antibody,clone OTI5C12. Catalog# M07004-1. Tested in IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-PTK9 (TWF1) Mouse Monoclonal Antibody [Clone ID: OTI5C12] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07004-1)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI5C12
Isotype
IgG2b
Immunogen
Human recombinant protein fragment corresponding to amino acids 140-384 of human TWF1 (NP_002813) produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M07004-1 is reactive to TWF1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
40.283kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07004-1 is guaranteed for IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TWF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TWF1 (1:2000).
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TWF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TWF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TWF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TWF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TWF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TWF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TWF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TWF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TWF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TWF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TWF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-TWF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
Click image to see more details
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TWF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
Click image to see more details
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TWF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
Click image to see more details
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and TWF1-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-TWF1 monoclonal antibody M07004-1 (1:2000). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Protein Target Info & Infographic
Gene/Protein Information For TWF1 (Source: Uniprot.org, NCBI)
Gene Name
TWF1
Full Name
Twinfilin-1
Weight
40.283kDa
Superfamily
actin-binding proteins ADF family
Alternative Names
A6 protein tyrosine kinase; A6; Protein A6; Protein tyrosine kinase 9MGC23788; PTK9 protein tyrosine kinase 9; PTK9MGC41876; twinfilin, actin-binding protein, homolog 1 (Drosophila); twinfilin-1 TWF1 A6, PTK9 twinfilin actin binding protein 1 twinfilin-1|A6 protein tyrosine kinase|PTK9 protein tyrosine kinase 9|protein A6|protein tyrosine kinase 9|twinfilin, actin-binding protein, homolog 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TWF1, check out the TWF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TWF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PTK9 (TWF1) Mouse Monoclonal Antibody [Clone ID: OTI5C12] (M07004-1)
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